ISSN:
0741-0581
Keywords:
Immunoelectron microscopy
;
Cryoultramicrotomy
;
Monoclonal antibodies
;
Striated muscle
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
A systematic approach to the discovery of new proteins of ultrastructural interest is discussed. It involves the merging of monoclonal antibody technology with immunocytochemical technology, particularly immunoelectron microscopy. In this approach, monoclonal antibodies are raised to a cellular preparation that can be grossly heterogeneous in its protein composition. The hybridoma culture fluids are screened by immunocytochemistry for the ultrastructural localization of their antibodies. Those monoclonal antibodies that show specific ultrastructural localizations of interest are then selected for further investigation. The antigen to which a given monoclonal antibody is directed is then identified by immunoprecipitation and immunoblotting with that antibody. By this approach, two new striated muscle proteins of ultrastructural interest have been discovered and are named zeugmatin and enactin. The former is a protein of over 500 kD localized by immunoelectron microscopy to the Z-bands, the latter of 245 kD localized to the N1 line of striated muscle.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060060213
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