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  • 1
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    Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells (1988)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1988-07-01
    Description: High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant approximately equal to 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-beta messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Komm, B S -- Terpening, C M -- Benz, D J -- Graeme, K A -- Gallegos, A -- Korc, M -- Greene, G L -- O'Malley, B W -- Haussler, M R -- New York, N.Y. -- Science. 1988 Jul 1;241(4861):81-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, University of Arizona College of Medicine, Tucson 85724.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3164526" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Binding, Competitive ; Cell Nucleus/metabolism ; DNA/genetics ; Estradiol/*metabolism/pharmacology ; Humans ; Iodine Radioisotopes ; Nucleic Acid Hybridization ; Osteoblasts/drug effects/*metabolism ; Osteosarcoma/*metabolism ; Peptides/genetics ; Procollagen/genetics ; RNA, Messenger/*metabolism ; Rats ; Receptors, Estrogen/genetics/*metabolism ; Transcription, Genetic ; Transforming Growth Factors ; Tumor Cells, Cultured
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Molecular cloning of the chicken progesterone receptor (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-08-15
    Description: To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct lambda gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longest cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Conneely, O M -- Sullivan, W P -- Toft, D O -- Birnbaumer, M -- Cook, R G -- Maxwell, B L -- Zarucki-Schulz, T -- Greene, G L -- Schrader, W T -- O'Malley, B W -- New York, N.Y. -- Science. 1986 Aug 15;233(4765):767-70.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2426779" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; Base Sequence ; Chickens ; *Cloning, Molecular ; Cross Reactions ; DNA/*metabolism ; Epitopes/analysis ; Female ; *Genes ; Humans ; Nucleic Acid Hybridization ; Oviducts/metabolism ; RNA, Messenger/genetics ; Receptors, Progesterone/*genetics ; Species Specificity
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
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