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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine geophysical researches 9 (1988), S. 265-290 
    ISSN: 1573-0581
    Keywords: electromagnetics ; permafrost ; offshore exploration ; electrical anisotropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A novel variation of the geophysical technique known as MOSES, for Magnetometric Off-Shore Electrical Sounding, has been developed to map the electrical properties of the sea floor in Aretic regions. The particular target is the permafrost layer under the Beaufort Sea, a layer containing frozen or partially frozen sediment from 100 to 600 m thick underlying shallow sea water, typically 10 to 100 m deep, and several tens of metres of soft sediment. A detailed knowledge of the location and physical properties of the permafrost layer is essential for accurate interpretation of reflection seismic data. The permafrost can contain pockets, regions or layers of gas hydrate. The latter is both a possible resource and a hazard to drilling operations or hydrocarbon production. A local map of the permafrost zone is essential geotechnical information required prior to the construction of an offshore structure or pipeline. The MOSES method is particularly suitable for offshore electrical mapping as it can be made relatively insensitive to the shielding effects of the highly conductive sea water, in sharp contrast to many other electrical techniques. The transmitter is a vertical, long-wire bipole, extending from the sea surface to the sea floor. A commutated current is fed to two large electrodes: one near the sea surface and the other on the sea floor. The return current is through the sea water and the subjacent sediment. The receiver consists of two horizontal orthogonal coils located on the sea floor, and the data are measurements of two components of the magnetic field as a function of frequency and transmitter-receiver horizontal separation. The electrical conductivity of a sample of frozen material is much smaller than that of unfrozen or partially frozen sediment of the same type. Frozen and unfrozen thin layers are often observed sequentially throughout the geological section. The resistivity measured as a function of depth by an electrical logging tool is consequently highly variable. The resulting depth-averaged resistivity, the resistivity resolved by a surface electrical method, is macro-an-isotropic. An experimental design study reveals that both the vertical and horizontal averaged resistivities could be determined in a MOSES sounding without vertical scale distortion. A test of the methodology in very shallow water was conducted in the spring of 1986 at a site, approximate coordinates (70° N, 134.5° W), 85 km north-west of the town of Tuktoyaktuk. The instrumentation was lowered and subsequently recovered through holes in the ice which covers the Beaufort Sea at that time of the year. The transmitter power was obtained from a single lead-acid battery. Transmitter-receiver separations ranged from 10 to 300 m. A rapid increase in sediment resistivity with depth was observed. The higher resistivity values are consistent with those expected for a partially frozen zone.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Glutathione S-transferase ; Herbieide detoxification ; Suspension culture ; Zea (herbicide detoxification)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The metabolism of the s-triazine herbicide atrazine has been compared in Zea mays seedlings and cell suspension cultures. The rapid detoxification observed in the shoots of whole plants was not seen in the cultured cells. This difference in metabolism could be accounted for by the varying substrate specificities of the isoenzymes of glutathione S-transferase (EC 2.5.1.18) present in the plant and the cells. A single form of the enzyme isolated from leaf tissue conjugated both atrazine and the chloracetanilide herbicide metolachlor. However, the two isoenzymes present in suspension-cultured cells although active against metolachlor, showed no activity toward atrazine. Following purification, the major form of transferase present in the cells was physically similar to the enzyme isolated from leaf (Mr=55000). Both proteins were dimers of subunit Mr=26300, and with isoelectric points in the range pH 4.3-4.9. The minor form of the enzyme present in culture showed a greater specificity for metolachlor than the major species. In addition the overall activity and ratio of the two isoenzymes varied over the culture growth cycle. These findings illustrate the need for characterizing enzymes involved in herbicide detoxification in plant cell cultures.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 25 (1987), S. 671-686 
    ISSN: 1573-4927
    Keywords: Trisomy ; chromatin ; average nucleosome size ; mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The chromatin of a mouse that is trisomic for part of chromosome 7 was investigated. Chromatin from trisomic tissue has a smaller average nucleosome DNA repeat length than chromatin from tissue taken from normal diploid littermates. DNA of the nucleosome cores is the same size in both normal and trisomic tissues. Not all of the nucleosome monomers have different repeat lengths. Normal and trisomic mouse kidney cells in tissue culture maintained their nucleosome repeat-length differences.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 171 (1987), S. 144-144 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 175 (1988), S. 99-106 
    ISSN: 1432-2048
    Keywords: Detoxification (herbicide) ; Enzyme induction ; Glutathione S-transferase ; Herbicide antidote ; Zea (herbicide detoxification)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An antiserum to glutathione S-transferase (EC 2.5.1.18) from maize (Zea mays L.) responsible for herbicide detoxification has been raised in rabbit. The antiserum was specific to the Mr 26000 subunit of the enzyme from maize seedlings and suspension-cultured cells, and recognized the isoenzymes active toward both atrazine and metolachlor. When plants were treated for 24 h with the herbicide antidote N,N-diallyl-2-2-dich-loroacetamide (DDCA), enzyme activities toward metolachlor were doubled in the roots and this was associated with a 70% increase in immunodetectable protein. Translation of polysomal RNA in vitro showed that the increase in the transferase in root tissue was brought about by a ninefold increase in mRNA activity encoding the enzyme. Treatment of suspension-cultured cells with cinnamic acid, metolachlor and DDCA raised enzyme activities but did not increase synthesis of glutathione S-transferase. In cultured maize cells, enzyme synthesis was maximal in mid-logarithmic phase, coinciding with the highest levels of enzyme activity. When callus cultures were established from the shoots of a maize line known to conjugate chloro-s-triazines, enzyme activity towards atrazine was lost during primary dedifferentiation. However, levels of total immunodetectable enzyme and activity toward metolachlor were increased in cultured cells compared with the parent shoot tissue.
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  • 6
    ISSN: 1432-1424
    Keywords: K+ channel ; ATP ; ATP4− ; ADP3− ; RINm5F cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The31P-NMR technique has been used to assess the intracellular ratios and concentrations of mobile ATP and ADP and the intracellular pH in an insulin-secreting cell line, RINm5F. The single-channel current-recording technique has been used to investigate the effects of changes in the concentrations of ATP and ADP on the gating of nucleotide-dependent K+ channels. Adding ATP to the membrane inside closes these channels. However, in the continued presence of ATP adding ADP invariably leads to the reactivation of ATP-inhibited K+ channels, even at ATP4−/ADP3− concentration ratios greater than 7∶1. Interactions between ATP4− and ADP3− seem competitive. An increase in the concentration ratio ATP4−/ADP3− consistently evoked a decrease in the open-state probability of K+ channels; conversely a decrease in ATP4−/ADP3− increased the frequency of K+ channel opening events. Channel gating was also influenced by changes in the absolute concentrations of ATP4− and ADP3−, at constant free concentration ratios. ADP-evoked stimulation of ATP-inhibited channels did not result from phosphorylation of the channel, as ADP-β-S, a nonhydrolyzable analog of ADP, not only stimulated but enhanced ADP-induced activation of K+ channels, in the presence of ATP. Similarly, ADP was able to activate K+ channels in the presence of two nonhydrolyzable derivatives of ATP, AMP-PNP and βγmethylene ATP.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5117
    Keywords: pH ; aluminium ; drift ; macroinvertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 24 h experimental episodes were created in a soft-water stream in upland Wales, by the simultaneous addition at separate points of sulphuric acid, aluminium sulphate and citric acid. In an upstream reference zone (A) the pH remained above 7, while in the treatment zones, B, C and D successively downstream, it was reduced to c. pH 4.9. Concentrations of filterable aluminium were 0.05 mg l-1 in Zone B, 0.27 mg l-1 (0.11 mg l-1 ‘labile’) in Zone C, and 0.23 mg l-1 (0.00 mg l-1 ‘labile’) in Zone D. Chemical manipulation coincided with a spate, during which flow increased from 0.02 m3s -1 to a maximum of 0.07 m3s-1. Only the ephemeropteran B. rhodani showed a drift response: drift density was not affected by flow (Zone A) or by organically bound aluminium (Zone D), but increased approximately 6-fold in both the acid (B) and ‘labile’ aluminium (C) zones; its benthic density declined significantly in Zones C and D, by 78% and 89% respectively. We relate these results to the biological importance of aluminium chemistry during natural acidic episodes.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 26 (1988), S. 175-185 
    ISSN: 1741-0444
    Keywords: Blood flow ; Computing ; Fluid mechanics ; Model ; Prosthetic heart valve design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The paper aims to present the strengths and weaknesses of computational fluid mechanics (CFM) compared with existing in vitro haemodynamic techniques such as LDA, hot-wire anemometry and static pressure measurement, and to discuss the application of CFM to the analysis of prosthetic heart valve flow. It presents a brief summary of existing methods used in analysing in vitro heart valve flow (along with CFM), the special problems inherent in each method and the quantities each is capable of giving. The analysis then goes on to present examples of information yielded by CFM. It also contains a brief discussion of the data requirements for the analysis of heart valve performance and the clinical relevance of fluid dynamic occurrences. The conclusion drawn is that CFM has a major role to play in the analysis of prosthetic heart valve flow and design because of the improved availability of data afforded and the increased correlation between CFM and clinical flow regimes, due to the ability to consider realistic models of blood.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 1988-11-01
    Print ISSN: 0018-8158
    Electronic ISSN: 1573-5117
    Topics: Biology
    Published by Springer
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  • 10
    Publication Date: 1987-10-01
    Print ISSN: 0006-2928
    Electronic ISSN: 1573-4927
    Topics: Biology , Chemistry and Pharmacology
    Published by Springer
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