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  • 1
    Electronic Resource
    Electronic Resource
    Aerosol transport of polynuclear aromatic hydrocarbons over the mediterranean sea (1988)
    Springer
    Naturwissenschaften 75 (1988), S. 39-42 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367439
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  • 2
    Electronic Resource
    Electronic Resource
    Complete minimal spheres and projective planes inR n with simple ends (1989)
    Springer
    Mathematische Zeitschrift 201 (1989), S. 375-380 
    Details
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01214902
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  • 3
    Electronic Resource
    Electronic Resource
    The Muridae glyceraldehyde-3-phosphate dehydrogenase family (1989)
    Springer
    Journal of molecular evolution 29 (1989), S. 212-222 
    Details
    ISSN: 1432-1432
    Keywords: Pseudogenes ; Amplification ; Retroposition ; Chromatin structure ; DNA methylation ; Transcription ; RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although only one gene is known to be functional, numerous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) related sequences are scattered throughoutMus musculus andRattus rattus genomes. In this report we show that: (1) GAPDH pseudogenes are repeated to comparable extents, at least 400 copies, in 12 other Muridae species; (2) the complete, or nearly so, sequence of GAPDH messenger RNA is amplified, and a high proportion, if not all of these copies, are intronless; (3) GAPDH pseudogenes are preferentially located in heavily methylated and DNAse I-insensitive regions of chromatin; and (4) the presence of atypical GAPDH-related mRNAs in different cellular contexts raises the possibility that more than one GAPDH gene is transcribed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100205
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  • 4
    Electronic Resource
    Electronic Resource
    Application of response surface methodology to optimization of glutaraldehyde activation of a support for enzyme immobilization (1985)
    Springer
    Applied microbiology and biotechnology 22 (1985), S. 88-91 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary We have studied the influence of several parameters (glutaraldehyde concentration, pH and contact time) on the activation of an amine silica with glutaraldehyde. In order to take interactions between parameters into consideration, we have used the Response Surface Methodology which allows us to obtain an equation (not a single estimation). The optimal conditions for glutaraldehyde activation of amine Spherosil beads were as follow: the porous silica was activated for 1 h at 25°C with an 8% (V/V) solution of glutaraldehyde at pH 6.4 these conditions were the same with RNA or CMP used as substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00250025
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  • 5
    Electronic Resource
    Electronic Resource
    A protein of Mr 80,000 is associated with the nucleolus organizer of human cell lines (1986)
    Springer
    Chromosoma 94 (1986), S. 353-361 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A rabbit serum which had previously been reported to have an immunological affinity for centrosomes of human cell lines was shown also to be specific for the nucleus. Optical and ultrastructural immunolocalization in HeLa cells showed that this specificity is restricted to the fibrillar centre of nucleoli either in untreated or actinomycin D treated interphase cells. In mitotic cells discrete labelling was observed on chromosomes and shown to correspond, on spread metaphase plates, to the short arms of acrocentric chromosomes, i.e. to the nucleolar organizer regions (NORs). Using independent cell fractionation procedures in the human T-lymphoblastic KE 37 cell line and purification of immunoglobulins by affinity to antigens detected by electrophoresis and blotting, a strict correlation between immunoreactive proteins and cytological staining was established. The nucleolar specificity was shown to correspond to a protein with an Mr of 80,000 while the centrosomal specificity corresponded principally to a protein doublet of 60,000–65,000. These antigens share common epitopes as shown by the staining of both NOR and centrosome by immunoglobulins purified by affinity to either type of protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328635
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  • 6
    Electronic Resource
    Electronic Resource
    9-amino-2-methoxy-6-chloroacridine monocation fluorescence analysis by phase-modulation fluorometry (1986)
    Springer
    European biophysics journal 13 (1986), S. 251-257 
    Details
    ISSN: 1432-1017
    Keywords: 9-amino-2-methoxy-6-chloroacridine monocation ; fluorescence lifetimes ; phase-modulation fluorometry ; solvent cage reorientation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The fluorescence of the 9-amino-2-methoxy-6-chloroacridinic monocation (ACMA) in various alcohol-water solutions was studied by phasemodulation fluorometry. Apparent phase and modulation lifetimes were determined at different observation wavelengths for three modulation frequencies. The results are explained by an orientational relaxation of the solvent cage subsequent to ACMA photoexcitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260372
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  • 7
    Electronic Resource
    Electronic Resource
    Oxide-dispersed titanium alloys Ti-Y prepared with the rotating electrode process (1987)
    Springer
    Journal of materials science 22 (1987), S. 887-895 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This work deals with an evaluation of the range of cooling rates during solidification necessary to obtain rare earth oxide-dispersed titanium alloys, which constitute a potentially interesting alloy system for high temperature use, due to the thermodynamic stability of rare earth oxides in titanium. The results of the microstructural studies on binary Ti-Y alloy powders prepared by the rotating electrode process show that it is possible to obtain e fine and homogeneous dispersion of yttrium oxide Y2O3 in a titanium maul with relatively moderate cooling rates (≈ 104 K sec−1). The results also indicate that the stability of the dispersion is excellent during hot consolidation but only in theα phase temperature region (〈 882.5° C). The preliminary mechanical teas performed on consolidated products show u substantial increase in 0.2% Woof stress (about 100 M Pa) up to 55O° C with respect to unalloyed titanium of commercial purity. Above 550° Q this strength increment becomes negligible or disappears. These results imply that the oxide dispersion loses its hardening effect at high temperatures, probably due to the increasing importance of grain-boundary sliding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103526
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  • 8
    Electronic Resource
    Electronic Resource
    Female settlement in marsh wrens: is it affected by other females? (1987)
    Springer
    Behavioral ecology and sociobiology 21 (1987), S. 135-140 
    Details
    ISSN: 1432-0762
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of resident females on the settling patterns of subsequent females was examined for marsh wrens (Cistothorus palustris). Proportionally more females responded to a playback of a female intruder during the pre-laying stage than during the laying and incubation stages. The strength of the response was also greatest during pre-laying and laying stages (Table 1). Female wrens did not settle randomly with respect to the presence of other females, but rather settled with bachelor males more often than predicted by chance (Table 3). Female settlement within territories was more asynchronous than settlement between territories. An attempt to fit these data to two models of female settlement suggested that prospecting females were not displaced by residents, but rather avoided settling with mated males (Table 3). Females within territories were also more dispersed than predicted by chance, a pattern that may be enforced through aggression on the part of the resident female. Staggered settlement reduces the overlap in nestling stages of harem mates and so may be a strategy to reduce competition for resources at this critical stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395442
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  • 9
    Electronic Resource
    Electronic Resource
    Female settlement in marsh wrens: is it affected by other females? (1987)
    Springer
    Behavioral ecology and sociobiology 21 (1987), S. 135-140 
    Details
    ISSN: 1432-0762
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of resident females on the settling patterns of subsequent females was examined for marsh wrens (Cistothorus palustris). Proportionally more females responded to a playback of a female intruder during the pre-laying stage than during the laying and incubation stages. The strength of the response was also greatest during pre-laying and laying stages (Table 1). Female wrens did not settle randomly with respect to the presence of other females, but rather settled with bachelor males more often than predicted by chance (Table 3). Female settlement within territories was more asynchronous than settlement between territories. An attempt to fit these data to two models of female settlement suggested that prospecting females were not displaced by residents, but rather avoided settling with mated males (Table 3). Females within territories were also more dispersed than predicted by chance, a pattern that may be enforced through aggression on the part of the resident female. Staggered settlement reduces the overlap in nestling stages of harem mates and so may be a strategy to reduce competition for resources at this critical stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553184
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  • 10
    Electronic Resource
    Electronic Resource
    Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 49-57 
    Details
    ISSN: 1617-4623
    Keywords: nifU ; nifS ; nifV ; nifW ; nifZ ; nifM ; Azotobacter vinelandii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Azotobacter vinelandii genes contained within the major nif-cluster and designated orf6, nifU, nifS, nifV, orf7, orf8, nifW nifZ, nifM, and orf9 are organized into at least two overlapping transcriptional units. Nitrogenase derepressed crude extracts of Azotobacter vinelandii mutant strains having individual deletions located within nifU, nifS, nifV, nif, nifZ, or nifM were examined for nitrogenase component protein activities. The results of these experiments indicated that, in A. vinelandii, the nifU, nifS and nifM gene products are required for the full activation or the catalytic stability of the nitrogenase Fe protein. Deletion of the nifV gene resulted in lower MoFe protein activity, probably resulting from the accumulation of an altered FeMo-cofactor. The nifW and nifZ gene products were required for the full activation or catalytic stability of the MoFe protein. Deletion of nijZ alone or nifM alone did not appear to affect FeMo-cofactor biosynthesis. However, deletion of both niJZ and nifM eleminated either FeMo-cofactor biosynthesis or the insertion of FeMo-cofactor into the apo-MoFe protein. Other genes contained within the nifUSVWZM gene cluster (orf6, orf7, orf8, and orf9) were not required for Mo-dependent diazotrophic growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261156
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