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  • Articles  (2)
  • Gene expression  (1)
  • Temperature  (1)
  • Springer  (2)
  • 1985-1989  (2)
Collection
  • Articles  (2)
Publisher
  • Springer  (2)
Years
  • 1985-1989  (2)
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Topic
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 123 (1985), S. 610-623 
    ISSN: 1420-9136
    Keywords: Radiative-convective model ; Cloud feedback ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The effect of cloud feedback on the response of a radiative-convective model to a change in cloud model parameters, atmospheric CO2 concentration, and solar constant has been studied using two different parameterization schemes. The method for simulating the vertical distribution of both cloud cover and cloud optical thickness, which depends on the relative humidity and on the saturation mixing ratio of water vapor, respectively, is the same in both approaches, but the schemes differ with respect to modeling the water vapor profile. In scheme I atmospheric water vapor is coupled to surface parameters, while in scheme II an explicit balance equation for water vapor in the individual atmospheric layers is used. For both models the combined effect of feedbacks due to variations in lapse rate, cloud cover, and cloud optical thickness results in different relationships between changes in surface temperature, planetary temperature, and cloud cover. Specifically, for a CO2 doubling and a 2% increase in solar constant, in both models the surface warming is reduced by cloud feedback, in contrast to no feedback, with the greater reduction in scheme I as compared to that of scheme II.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 12 (1987), S. 527-534 
    ISSN: 1432-0983
    Keywords: Fission yeast ; OMPdecase ; Cloning ; Gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in an Escherichia coli-Schizosaccharomyces pombe (E. coli-S. pombe) replicative plasmid to a 1.76 kb HindIII fragment. This plasmid is maintained at a high copy number in S. pombe and allows OMPdecase expression in Saccharomyces cerevisiae (S. cerevisiae) as well as in E. coli. After characterisation by restriction mapping and Southern hybridisation, the cloned gene was used as a probe to measure URA4 transcription and to examine its regulation. Messenger RNA levels were measured by DNA/RNA filter-hybridisation with pulse labelled RNAs during 6-azauridine (6-AUR) inhibited growth in wild type and 6-AUR sensitive strains. We found that in S. pombe the OMP analogue 6-AUR does not regulate the level of OMPdecase formation as it does in S. cerevisiae but rather modifies the ratio of total polyA+ to polyA− RNAs in the cell. Based on these results and on corresponding enzyme activities this study demonstrates divergent pyrimidine pathway regulation in the two yeasts S. cerevisiae and S. pombe. Finally, we propose the use of the URA4 gene as a convenient selective marker for genetic engineering in S. pombe.
    Type of Medium: Electronic Resource
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