ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Serum carotenoids
;
Amino-cyano stationary phases
;
Gradient Elution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Polarities of the carotenoids in human serum are very different; many nonpolar carotenoid hydrocarbons (e.g. β-carotene, lycopene) and highly polar hydroxycarotenoids (e. g. β-cryptoxanthin, zeaxanthin, lutein) can be found among them. Gradient elution chromatography was used for the separation of β-carotene, lycopene, β-cryptoxanthin, lutein and zeaxanthin in serum samples applying amino and cyano packings (Chromsil-NH2, Chromsil-CN). The effects of different stationary phases on the selectivity were compared. The method is particularly suitable for the direct determination of serum levels of carotenoid in serum extracts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02257325
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