ISSN:
1432-2242
Schlagwort(e):
Wheat endosperm
;
α-amylase inhibitors
;
Gene mapping
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary Three α-amylase inhibitors, designated Inh. I, II and III have been purified from the 70% ethanol extract of hexaploid wheat (Triticum aestivum L.) and characterized by amino acid analysis, N-terminal amino acid sequencing and enzyme inhibition tests. Inhibitors I and III have identical N-terminal sequences and inhibitory properties to those of the previously described 0.19/0.53 group of dimeric inhibitors. Inhibitor II has an N-terminal sequence which is identical to that of the previously described 0.28 monomeric inhibitor, but differs from it in that in addition to being active against α-amylase from Tenebrio molitor, it is also active against mammalian salivary and pancreatic α-amylases. Compensating nulli-tetrasomic and ditelosomic lines of wheat cv. ‘Chinese Spring’ have been analysed by two-dimensional electrophoresis, under conditions in which there is no overlap of the inhibitors with other proteins, and the chromosomal locations of the genes encoding these inhibitors have been established: genes for Inh. I and Inh. III are in the short arms of chromosomes 3B and 3D, respectively, and that for Inh. II in the short arm of chromosome 6D.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00261464
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