ISSN:
1617-4623
Keywords:
2 μm plasmid
;
Maintenance
;
Partitioning
;
Gene function
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Stable maintenance of the yeast 2 μm circle requires an origin of replication and three additional plasmidencoded functions. These are the two trans-acting products of plasmid genes B and C, and the cis-acting region designated STB or REP3. We have carried out detailed studies of the mitotic stability and copy number of 2 μm-based plasmids in [cir+] and [cir0] hosts. The results obtained demonstrate that all three of these functions play a major role in plasmid partitioning. The behaviour of hybrid plasmids carrying various regions of the 2 μm circle has also been studied in [cir0] isolates containing chromosomally integrated copies of the B and/or C genes. The results demonstrate that the efficiency of partitioning is dependent upon the dosage of gene B but is independent of the dosage of gene C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00330397
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