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  • Articles  (3)
  • Anesthesia  (1)
  • Antigen distribution  (1)
  • Hymenoptera
  • IBA
  • Springer  (3)
  • National Academy of Sciences
  • 1985-1989  (3)
Collection
  • Articles  (3)
Publisher
  • Springer  (3)
  • National Academy of Sciences
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 240 (1985), S. 417-423 
    ISSN: 1432-0878
    Keywords: Cysteine protease ; Epidermal cells ; Antigen localization ; Cell differentiation ; Antigen distribution ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monospecific antibody directed to cysteine protease of 2-day-old rat epidermis recently characterized as being different from the proteases previously reported was produced in rabbits. By immunofluorescence microscopy and immunoperoxidase staining with an avidin-biotin-peroxidase method the protease was found to be present in the epidermis of rodents of different ages as well as that of humans, but not in the dermis. The staining in germinative cells was more intense than in cells in the superficial layers. It appeared as irregular patches in the nuclei and stained more diffusely in the cytoplasm where small granular components, strongly stained, were identified. The staining patterns in granular cells showed accumulation of the antigen in a granular form. The morphology and distribution of granules resembled those of keratohyalin-like granules in the nucleus and dense homogenous deposits in the cytoplasm. In cornified cells the reaction product was localized by the plasma membrane where concentration of the dense homogenous deposits occurred, suggesting that the cysteine protease is one component of the unique and characteristic structure of differentiated keratinocytes. In addition, the cysteine protease antigen having the same molecular weight as the epidermal enzyme was detected in liver, kidney and lung indicating a wider tissue distribution of the protease. The significance of the protease in regulation of cellular functions remains to be investigated.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant growth regulation 8 (1989), S. 215-223 
    ISSN: 1573-5087
    Keywords: IAA ; IBA ; conjugated IBA ; rooting ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-butyric acid (IBA) was identified by thin layer chromatography, gas-liquid chromatography and gas chromatography-mass spectrometry in kernels and leaves of corn (Zea mays) var. Hazera 224. Free and ester conjugated IBA were present in dry and germinating corn kernels and leaves. This is the first report of IBA in a monocotyledonous plant and, as far as we know, the first evidence for the presence of conjugated IBA.
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  • 3
    ISSN: 1573-2614
    Keywords: Monitoring ; blood pressure ; Equipment ; noninvasive blood pressure monitors ; Measurement techniques ; blood pressure ; Anesthesia ; regional ; spinal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Medicine
    Notes: Abstract A noninvasive blood pressure monitor (Finapres) that continuously displays the arterial waveform using the Penňaz methodology has recently been introduced into clinical practice. We compared this device with an automated oscillometric blood pressure monitor (Dinamap 1846SX) in 20 patients during spinal anesthesia for nonemergency cesarean section according to a procedure suggested by the Association for the Advancement of Medical Instrumentation. After administration of the spinal anesthetic, the Finapres monitor produced systolic, mean, and diastolic pressure measurements greater than those of the Dinamap monitor (6.6±12.5, 3.3±10.4, and 7.2±9.8 mm Hg, respectively). In most patients, the Finapres measurements were similar to those determined by the Dinamap; however, in 4 patients, mean systolic differences were greater than 20 mm Hg. These patients did not differ from the others in age, height, weight, or baseline blood pressure, and the pressure values recorded by the Finapres monitor were substantially higher than those measured by auscultation in the labor room. In 30% of the patients, the offset between Dinamap and Finapres blood pressure measurements changed markedly over the course of the surgical procedure. The Finapres monitor occasionally stopped working and had to be restarted. In 1 patient (not included in this analysis), the Dinamap monitor was unable to determine the blood pressure due to patient shivering; this did not appear to interfere with the Finapres. We conclude that the Finapres monitor does not consistently provide blood pressure information equivalent to that of the Dinamap in obstetric patients undergoing spinal anesthesia. When the Finapres monitor is used, pressure measurements should be verified periodically by using an auscultatory or oscillometric blood pressure methodology to rule out the presence of large differences, particularly in systolic pressure. The extreme systolic blood pressure discrepancies noted in 20% of the patients studied warrant further evaluation.
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