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  • Wiley-Blackwell  (5,496)
  • 1985-1989  (5,496)
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Keywords
Publisher
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Year
  • 1
    Electronic Resource
    Electronic Resource
    A comparison of chromatographic and chromatographic/mass spectrometric techniques for the determination of polycyclic aromatic hydrocarbons in marine sedimentsNRCC No. 26728. (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 14 (1987), S. 375-381 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The establishment of reliable values for concentrations of 16 priority pollutant polycyclic aromatic hydrocarbons (PAH) in a suite of marine sediment reference materials included an examination of the methods used to determine such compounds. Results from the five techniques used indicate large method biases. The biases arise from chemical interferences in methods which use non-selective measuring systems with separation techniques which do not first completely resolve the analytes; e.g., single-parameter optical detectors with high performance liquid chromatographic (HPLC) techniques or a flame ionisation detector (FID) with capillary column gas chromatographic (GC) methods. Measurement by a mass spectrometer with HPLC and GC removes much of the method bias. Results for a representative sediment are discussed to illustrate these conclusions.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200140805
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  • 2
    Electronic Resource
    Electronic Resource
    Measurement of oxygen solubility in fermentation media: A colorimetric method (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 578-583 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Methods of measuring oxygen solubility in culture media are scarce, and those available are tedious to apply. A simple colorimetric assay was developed and applied to the analysis of oxygen solubility during alcoholic fermentation. The method was based on the consumption of oxygen by glucose oxidase activity and the production of the pink quinone of syringaldazine by coupled peroxidase activity. Color formation at 526 nm progressed through an optimum that was a linear function of the oxygen added to the assay. Sensitivity was maximized by operating at pH 7 and limiting the medium sample volume added. Each assay took 10-15 min to prepare and react. Reaction time was minimized by using abundant glucose and enzyme concentrations. Data obtained by the assay developed showed good agreement with published oxygen solubilities in water and selected media at various temperatures. Subsequent analyses of fermentation broths indicated falling sugar concentration to be primarily responsible for increases in oxygen solubility during fermentation. For example, during fermentations started with 230 g/L xylose or glucose, oxygen solubility could increase by 41% due to sugar consumption alone. This procedure can provide the solubility data needed to accurately calibrate in-line electronic probes for monitoring dissolved oxygen concentration during fermentation processes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260330510
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  • 3
    Electronic Resource
    Electronic Resource
    w4-Mutable line in soybean (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 10 (1989), S. 542-551 
    Details
    ISSN: 0192-253X
    Keywords: Glycine max ; Transposable element ; Transposon tagging ; Genetic instability ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: An unstable mutation for anthocyanin pigmentation in soybean (Giycine max [L.] Merr.) was identified in 1983. The mutability is conditioned by an allele at the w4 locus that is recessive to wild type. The population containing the mutable allele is known as the w4-mutable line. Most plants in the line have chimeric flowers with purple sectors on a near-white background. The mutable allele yields germinal revertants at a rate that varies from 5 to 10% per generation, and the revertant alleles are stable. Approximately 1% of the progenies derived from germinal revertant plants contain mutations at other loci These features, as well as the occurrence of pale flower phenotypes and changes of state, suggest that a transposable element system is producing the unstable phenotype.Several new mutants were isolated in an experiment designed to tag loci. The first three chlorophyll-deficient mutants found (CD-1, CD-2, and CD-3) are inherited as single-gene recessives. Each of the mutants lacks the same two mitochondrial malate dehydrogenase (MDH) bands. No recombination has been detected between the MDH phenotype and the chlorophyll-deficient phenotype. Genetic data indicate that the three mutants are allelic, and additional evidence suggests that each of the CD mutants is the result of a deletion. In the CD-1, CD-2, and CD-3 mutants, the deletions result in the silencing of an MDH locus, atypical chloroplast development, and an altered chlorophyll composition. Additional mutants for root necrosis, partial and near sterility, chlorophyll deficiency, and flower color isolated from the transposon tagging study have provided material for future research.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020100613
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  • 4
    Electronic Resource
    Electronic Resource
    The combined use of NMR, distance geometry, and restrained molecular dynamics for the conformational study of a cyclic somatostatin analogue (1988)
    New York : Wiley-Blackwell
    Biopolymers 27 (1988), S. 323-338 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A cyclic peptide analogue of somatostatin, including the o-aminomethylphenylacetic acid spacer, was studied by the combined use of two-dimensional nmr spectroscopy, distance geometry, and restrained molecular dynamics. Analysis of distances determined from nuclear Overhauser effect (NOE) buildup rates revealed that these were inconsistent with a unique backbone conformation near the spacer. Assuming that the conformational heterogeneity is localized to the spacer, the NOE distances measured for the remaining part of the molecule were used to generate a large number of structures with the distance geometry algorithm, which were then refined by restrained energy minimization. Four classes of structures emerged, which together account for all observed NOEs. A representative structure of each class was further refined with the restrained molecular dynamics technique, and shown to be stable on a 20-ps time scale. The flexibility of the spacer was examined by simulating interconversions induced by an appropriate restraining potential. As a result, the explanation for the lack of somatostatin activity of the analogue studied was reconsidered.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360270211
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  • 5
    Electronic Resource
    Electronic Resource
    Two-dimensional 1H-nmr studies on the lac repressor DNA binding domain: Further resonance assignments and identification of nuclear overhauser enhancements (1985)
    New York : Wiley-Blackwell
    Biopolymers 24 (1985), S. 2257-2277 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The assignments of several 1H lines in the spectrum of the lac repressor DNA-binding domain (or “headpiece”) are documented. These new assignments complement those obtained in a previous study [E.R.P. Zuiderweg, R. Kaptein, and K. Wüthrich (1983) Eur. J. Biochem. 137, 279] and were made from pure absorption phase two-dimensional nuclear Overhauser enhancement (2D NOE) spectra and 1H 2D double-quantum spectra of this protein. Based on the complete set of resonance assignments, a large number of interresidue NOEs are identified; these are documented and their relation with distance constraints is discussed. The identification of these NOEs is a prerequiste for the determination of the spatial structure of this protein, for which no crystallographic data are available.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360241208
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular orientation of polypropylene by rolling-drawing (1988)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 28 (1988), S. 1264-1269 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Rolling-drawing is a simple, effective, solid state processing technique for manufacturing high strength and high modulus oriented polymer sheet products. The process is capable of increasing the tensile modulus and strength of polypropylene by more, than an order of magnitude with inexpensive equipment and straight forward controllable techniques. This paper gives an overview of the rolling-drawing of polypropylene. It is intended to answer the following questions. What is rolling-drawing? What changes in tensile properties can be expected as a result of this process? What deformation processes occur during rolling-drawing? What are the processing variables and how do these variables relate to the deformation ratio achieved by rolling-drawing? A tensile flow stress relationship was formulated from experimental data for oriented polypropylene. This constitutive equation, estimates the flow stress (or yield stress) of the polymer as a function of deformation ratio, strain rate and temperature. Since stretching was found to provide a significant portion of the deformation during the rolling-drawing process a Hoffman-Sachs computer analysis was written to predict plastic deformation and drawing forces in the stretch zone. The results of experimentation and the analysis are briefly compared in this paper.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760281908
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  • 7
    Electronic Resource
    Electronic Resource
    Identification of metabolites of tiropramide in human urine (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 205-209 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The metabolites of tiropramide were extracted from the urine of healthy volunteers given tiropramide hydrochloride orally. Eight metabolites of tiropramide were found by gas chromatography/mass spectrometry. Three were identified on the basis of their mass spectra, retention times and comparison with synthetic standards. For the others a probable structure is proposed on the basis of their mass spectra.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200150404
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  • 8
    Electronic Resource
    Electronic Resource
    Structural analysis of resin developed as an adhesive for poly(w-aminoundecanoic acid) (1986)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 24 (1986), S. 3573-3575 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1986.080241237
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular factors affecting solubility in rigid-rod polyamides (1987)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 25 (1987), S. 1249-1271 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The solubility of a series of para-linked, substituted phenyl, biphenyl, terphenyl, and quaterphenyl polyamides is reported. Most of these polymers are soluble in amide solvents without lithium chloride, and several are soluble in ethers and/or ketones. The results indicate that solubility in this class of polymers is affected by the position, polarizability and size of the substituents, enantiomeric purity, and the number of non-coplanar biphenyl rings per repeat unit.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1987.080250505
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  • 10
    Electronic Resource
    Electronic Resource
    Anionic syntheses of terminally functionalized ethylene oligomers (1989)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 27 (1989), S. 4205-4226 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Synthetic procedures for preparation of terminally functionalized linear ethylene oligomers are described. The preferred synthetic method is anionic oligomerization of ethylene with n-butyllithium-tetramethylethylenediamine and electrophilic substitution of the living oligomer so-formed. Conditions and procedures for subsequent chemistry to elaborate the end groups of these oligomers are described. These procedures afford strictly linear ethylene oligomers which contain a wide variety of end groups and which range in molecular weight from 1000 to 4500 (Mn). The product oligomers were characterized spectroscopically as toluene-d8 solutions at 110°C using multinuclear NMR, FT-IR, fluorescence, and UV-visible spectroscopies as appropriate. Alternative stepwise approaches to such oligomers are also discussed.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1989.080271226
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