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  • Blackwell Publishing Ltd  (6)
  • Oxford University Press
  • 1985-1989  (6)
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Publisher
Years
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  • 1
    Electronic Resource
    Electronic Resource
    An Indo-Pacific trunkfish Tetrosomus gibbosus (Linnaeus): first record of the family Ostracionidae in the Mediterranean (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 32 (1988), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1988.tb05420.x
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  • 2
    Electronic Resource
    Electronic Resource
    GROWTH LAYER GROUPS (GLGs) IN THE TEETH OF AN ADULT BELUKHA WHALE (DELPHINAPTERUS LEUCAS) OF KNOWN AGE: EVIDENCE FOR TWO ANNUAL LAYERS (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 3 (1987), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Growth layer groups (GLGs) were counted in teeth from an adult male belukha whale (Delphinapterus leucas) that had lived for 23 yr in captivity and was estimated to have been 14 mo at capture. As defined here, GLGs are repeating areas of alternating opaque (denser) and translucent (less dense) dentin or cementurn, or nodes at the dentin-cementum interface. A GLG involves at least one change from opaque to translucent, dense to less dense or ridge to groove, but may be further subdivided by incremental growth layers or laminations. Teeth were prepared by two techniques. Thick sections (longitudinal half sections) were examined with a dissecting microscope under reflected light. Thinner cross and longitudinal sections were x-rayed and the plates scanned with a microdensitometer. Scanning electron photomicrographs were prepared from thick sections, but SEM proved to be the least useful technique. Counts of GLGs were variable because the laminations were numerous and some surface layers had been lost from wear. Thick- and thinner-section techniques gave comparable results, and approximately 40 GLGs were counted consistently. Although captive environments are less variable than natural ones, our findings are further evidence that belukhas in the wild deposit more than one—and probably two—GLGs per year.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.1987.tb00148.x
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  • 3
    Electronic Resource
    Electronic Resource
    Deposition of callose in relation to abscission of citrus leaves (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 72 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: When leaves of Citrus sinensis (L.) Osbeck cv. Shamouti senesce, they become more susceptible to abscission and the proximal 2 mm of their lamina-petiole abscission zones exhibit callose deposition. The degree of senescence, assayed with the DAR-WIN image processor (Telewski et al. 1983), was positively correlated in a linear fashion with callose deposition. Explant of non-senescing leaves were observed. Excision of the leaf at the stem-petiole junction induced callose deposition throughout the petiole, but not in the lamina. Callose deposition began immediately upon excision and reached a maximum at 3 h. It then decreased slightly and remained at the same level for up to 5 days. Exogenous compounds that decrease callose deposition, e.g. laminarase and 2-deoxy-D-glucose, inhibited the rate of abscission of explants. Compounds that promote callose deposition, e.g. uridine diphosphoglucose and mannose, increased the rate of abscission of explants. Exogenous callose, e.g. laminarin, increased the rate of abscission. It is not known how callose might be causally involved in promoting abscission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb05841.x
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of 2,5-norbornadiene on abscission and ethylene production in citrus leaf explants (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 63 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Citrus (Citrus sinensis L. Osbeck) leaf explants completely abscise within 48 h when exposed to saturating amounts of ethylene at 25°C. When 2,5-norbornadiene was added, 2000 μl 1−1 reduced abscission of explants also exposed to 2 μl 1−1 of ethylene to the level of the control, and 8000 μl 1−1 reduced abscission in explants exposed to 10 μl 1−1 of ethylene to the level of the control, but abscission was complete when 1 000 μl 1−1 of ethylene was used in the presence of 8 000 μl 1−1 of 2,5-norbornadiene. When explants were exposed to 2 μl 1−1 of ethylene, 2000 μl 1−1 of 2,5-norbornadiene prevented abscission if applied up to 10 h after exposure to ethylene. After 18 h, applied 2,5-norbornadiene had little effect on abscission at 48 h. A Lineweaver-Burk plot gave a 1/2 maximum value of 0.12 μl 1−1 for ethylene on abscission, 2,5-Norbornadiene gave competitive kinetics with respect to ethylene with a K1 value of approximately 120 μl 1−1 of 2,5-norbornadiene. The presence of 2,5norbornadiene stimulated ethylene production, which progressively increased as the 2,5-norbornadiene concentration was increased from 250 to 8 000 μl 1−1 2,5-Norbornadiene also suppressed the induction of cellulase and polygalacturonase by ethylene. Together, 2,5-norbornadiene and 2,4-dichlorophenoxyacetic acid were more effective than either alone in reducing abscission. 2,5-Norbornadiene also was effective in preventing the reduction of indole-3-acetic acid transport induced by ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb02828.x
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of different enzyme immunoassays for measuring indole-3-acetic acid in vegetative citrus tissues (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 68 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies, which were raised against indole-3-acetic acid (IAA) conjugated to bovine serum albumin (BSA) via the indolic nitrogen (IAA-N1-BSA), has been developed. The sensitivity and specificity of these antibodies were compared to those of polyclonal and monoclonal antibodies raised against IAA conjugated to BSA via C1 of the carboxyl group (IAA-C1-BSA). The sensitivity of the assays improved in the following order: monoclonal antibodies 〉 antibodies to IAA-C1-BSA 〉 antibodies to IAA-C1-BSA. Antibodies against IAA-C1-BSA had less cross-reactivity to indoles structurally related to IAA, excluding indole-3-pyruvic acid. A rapid and effective method for purification of IAA in citrus tissues before analysis by ELISA is described. Values of IAA in citrus (Citrus sinensis [L.] Osbeck cv. Shamouti orange) shoot tips obtained with all three antibodies were similar. However, in leaf tissues which contain lower amounts of IAA compared to shoot tips, monoclonal antibodies gave higher values of IAA than polyclonal antibodies. Estimation of free IAA levels in purified extracts of citrus shoot tips, very young leaves, and mature leaves was ca 380, 248, and 74 ng (g fresh weight)−1 respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb01924.x
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  • 6
    Electronic Resource
    Electronic Resource
    Invertase and sucrose synthase activity, carbohydrate status and endogenous IAA levels during Citrus leaf development (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 69 (1987), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Levels of activity of the sucrose catabolizing enzymes, acid invertase (EC 3.2.1.26) and sucrose synthase (EC 2.4.1.13), were measured during development of new leaves of Citrus sinensis (L.) Osbeck cv. Shamouti. Soluble acid invertase showed a peak activity of 32 nkat (g fresh weight)−1 at ca 60% of full leaf expansion and rapidly declined toward and after full expansion. There was no concomitant increase in an insoluble form of the enzyme. Sucrose synthase activity, measured in the synthesis direction, declined from 33% of full leaf expansion [10 nkat (g fresh weight)−1] 10, and following, full expansion. Highest sucrose synthase activity, measured in the cleavage direction, was 6 nkat (g fresh weight)−1 and showed little change during development. Acid invertase has a Km of 5 mMfor sucrose, while sucrose synthase had a Km of 118 mM for sucrose. Changes in acid invertase activity correlated with changes in the reducing sugar:sucrose ratio. These results suggest that soluble acid invertase activity is the primary enzyme responsible for sucrose catabolism in the expanding Citrus leaf. Changes in leaf expansion rate and invertase activity did not correlate positively with changes in endogenous free IAA level, as determined by enzyme linked immunoassay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1987.tb01959.x
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