ISSN:
1432-0878
Keywords:
ACTH
;
Cyanoketone
;
Δ53β
;
HSD activity
;
Interrenal glands
;
Rana catesbeiana
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The present communication describes an investigation of stimulation and inhibition of Δ5-3β-hydroxysteroid dehydrogenase in interrenal glands of tadpoles of Rana catesbeiana. Frozen sections of interrenal glands, together with kidneys, were prepared histochemically for assay of Δ5-3β-HSD activity. Concentrations of 0.01, 0.1, 1, and 10 IU/ml of ACTH or of 0.01, 0.1, 1, and 10 μg/ml of cyanoketone were added to the incubation media. The reaction products of the histochemically prepared slides, in terms of absorbance, were scanned at a defined area with a computerized microscope spectrophotometer. The results indicate that ACTH causes a significant dose-response stimulation of Δ5-3β-HSD activity in tadpole interrenals; cyanoketone, on the other hand, causes significant dose-dependent inhibition.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00218950
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