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  • ACTH  (1)
  • Chlamydia trachomatis  (1)
  • Springer  (2)
  • American Institute of Physics
  • American Institute of Physics (AIP)
  • 1985-1989  (2)
Collection
Publisher
  • Springer  (2)
  • American Institute of Physics
  • American Institute of Physics (AIP)
Years
  • 1985-1989  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 53 (1987), S. 191-196 
    ISSN: 1572-9699
    Keywords: Chlamydia trachomatis ; tetracycline ; susceptibility ; cell culture ; microtest ; inclusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel approach to determine the tetracycline susceptibility of Chlamydia trachomatis directly from specimens without cell culture propagation and adaptation has been explored. Out of a total of 1290 genital specimens from a sexually transmitted disease clinic, 211 (16.4%) were positive for C. trachomatis. A tetracycline concentration of 0.032 μg/ml completely inhibited the appearance of inclusions in all of the 211 positive specimens. Of the positive specimens, 120 (56.9%) and 18 (8.5%) respectively showed the presence of 1 to 9 and 100 or more inclusions per microtiter well in antibiotic free medium. Other antibiotics are being tested in the same manner.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: ACTH ; Cyanoketone ; Δ53β ; HSD activity ; Interrenal glands ; Rana catesbeiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present communication describes an investigation of stimulation and inhibition of Δ5-3β-hydroxysteroid dehydrogenase in interrenal glands of tadpoles of Rana catesbeiana. Frozen sections of interrenal glands, together with kidneys, were prepared histochemically for assay of Δ5-3β-HSD activity. Concentrations of 0.01, 0.1, 1, and 10 IU/ml of ACTH or of 0.01, 0.1, 1, and 10 μg/ml of cyanoketone were added to the incubation media. The reaction products of the histochemically prepared slides, in terms of absorbance, were scanned at a defined area with a computerized microscope spectrophotometer. The results indicate that ACTH causes a significant dose-response stimulation of Δ5-3β-HSD activity in tadpole interrenals; cyanoketone, on the other hand, causes significant dose-dependent inhibition.
    Type of Medium: Electronic Resource
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