ISSN:
1432-072X
Keywords:
Methane-oxidizing bacteria
;
Methylomonas rubra
;
Coenzyme Q
;
Lipids
;
Two-dimensional proton nuclear magnetic resonance spectrometry
;
Mass spectrometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The respiratory quinone composition of the obligate methane-utilizing bacterium Methylomonas rubra was examined. A single lipoquinone was isolated which on examination by thin-layer chromatography cochromatographed with coenzyme Q. Reverse-phase partition and argentation high performance liquid chromatography demonstrated the lipoquinone did not correspond to any known coenzyme Q prenologue. On the basis of mass spectrometry and proton nuclear magnetic resonance spectrometry the novel lipoquinone was shown to correspond to 2,3-dimethoxy-5-methyl-6-(11′-methylene-3′,7′,15′, 18′, 18′, 19′, 23′-heptamethyltetracosa-2′, 6′, 14′, 19′, 22′-pentaenyl-)-1,4-benzoquinone.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00403227
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