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  • 1
    Digitale Medien
    Digitale Medien
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 32 (1986), S. 193-200 
    ISSN: 0730-2312
    Schlagwort(e): hen oviduct ; human preplacental lactogen ; phosphlipid reactivation ; purification ; signal peptidase ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Signal peptidase has been purified approximately 600-fold from hen oviduct microsomes. Treatment of microsomes with ice-cold sodium carbonate at pH 11.5 removes soluble and extrinsic membrane proteins prior to solubilization of signal peptidase with Nonidet P-40. After dialysis to pH 8.2, the solubilized enzyme is chromatographed on diethylaminoethyl cellulose at pH 8.2. More than 90% of contaminating proteins bind to the column while signal peptidase and endogenous phospholipid are eluted in the column void volume. Enzyme activity subsequently binds to carboxymethyl cellulose at pH 5.8 and is eluted by approximately 100 to 200 mM NaCl during a NaCl gradient. Polypeptides present in partially purified hen oviduct signal peptidase have relative molecular masses ranging from 54 kD to less than 11 kD with major bands at 29, 23, 22, 19, 18 and 13 kD. The purified peptidase requires phospholipid for activity and is maximally active in the presence of 2 mg/ml phosphatidylcholine.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 9 (1985), S. 199-223 
    ISSN: 0363-9061
    Schlagwort(e): Engineering ; Engineering General
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Architektur, Bauingenieurwesen, Vermessung , Geologie und Paläontologie
    Notizen: A 3-D mathematical approach to slope stability, which is based on limiting equilibrium and variational analysis, is presented. In the initial formulation there are three unknown functions: the slip surface, the normal stress and the shear stress direction over this surface. The minimum factor of safety is sought through variational extremization. The analysis indicates that the factor of safety is independent of the normal stress distribution over the critical slip surface. It also indicates that the direction of the elementary shear force over the slip surface depends on the slip surface function, but not on the normal stress function. The analysis yields a non-linear first order partial differential equation, relating the slip surface and its first partial derivatives. By limiting the analysis to symmetrical problems an ordinary differential equation, governing the slip surface path on the plane of symmetry, is derived. This equation enables the development of a numerical procedure to determine the minimal factor of safety of symmetrical 3-D slopes. Two possible failure modes are determined for homogeneous slopes. One mode consists of finite 3-D sliding body and the second represents cylindrical failure. Numerical analyses for some simple cases, of homogencous slopes are presented.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 1986-01-01
    Print ISSN: 0149-1423
    Digitale ISSN: 1943-2674
    Thema: Geologie und Paläontologie
    Standort Signatur Erwartet Verfügbarkeit
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