Publication Date:
1986-05-09
Description:
A chimeric gene containing a cloned cDNA of the coat protein (CP) gene of tobacco mosaic virus (TMV) was introduced into tobacco cells on a Ti plasmid of Agrobacterium tumefaciens from which tumor inducing genes had been removed. Plants regenerated from transformed cells expressed TMV mRNA and CP as a nuclear trait. Seedlings from self-fertilized transgenic plants were inoculated with TMV and observed for development of disease symptoms. The seedlings that expressed the CP gene were delayed in symptom development and 10 to 60 percent of the transgenic plants failed to develop symptoms for the duration of the experiments. Increasing the concentration of TMV in the inoculum shortened the delay in appearance of symptoms. The results of these experiments indicate that plants can be genetically transformed for resistance to virus disease development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abel, P P -- Nelson, R S -- De, B -- Hoffmann, N -- Rogers, S G -- Fraley, R T -- Beachy, R N -- GM07067/GM/NIGMS NIH HHS/ -- GM08036/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1986 May 9;232(4751):738-43.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3457472" target="_blank"〉PubMed〈/a〉
Keywords:
DNA/genetics
;
DNA, Viral/genetics
;
*Genes, Viral
;
*Genetic Engineering
;
*Plant Diseases
;
Plants/*genetics/microbiology
;
Plants, Toxic
;
Rhizobium/genetics
;
Tobacco/genetics
;
*Tobacco Mosaic Virus
;
Viral Envelope Proteins/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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