Publication Date:
1988-09-02
Description:
The human ribosomal RNA promoter contains two distinct control elements (UCE and core) both of which are recognized by the sequence-specific DNA binding protein UBF1, which has now been purified to apparent homogeneity. The purified factor activates RNA polymerase I (RNA pol I) transcription through direct interactions with either control element. A second RNA pol I transcription factor, designated SL1, participates in the promoter recognition process and is required to reconstitute transcription in vitro. Although SL1 alone has no sequence-specific DNA binding activity, deoxyribonuclease I footprinting experiments reveal that a cooperative interaction between UBF1 and SL1 leads to the formation of a new protein-DNA complex at the UCE and core elements. In vitro transcription experiments indicate that formation of the UBF1-SL1 complex is vital for transcriptional activation by UBF1. Thus, protein-protein interactions between UBF1 and SL1 are required for targeting of SL1 to cis-control sequences of the promoter.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bell, S P -- Learned, R M -- Jantzen, H M -- Tjian, R -- GM 32856/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1988 Sep 2;241(4870):1192-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Biochemistry, University of California, Berkeley 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3413483" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Chromatography, Affinity
;
DNA/metabolism
;
DNA, Ribosomal/genetics
;
DNA-Binding Proteins/*metabolism
;
Deoxyribonuclease I/metabolism
;
HeLa Cells
;
Humans
;
Promoter Regions, Genetic
;
RNA Polymerase I/metabolism
;
RNA, Ribosomal/*biosynthesis
;
Transcription Factors/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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