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  • Column liquid chromatography  (85)
  • Ultrastructure  (49)
  • Springer  (134)
  • American Chemical Society
  • Elsevier
  • Institute of Physics
  • 1985-1989  (134)
  • 1940-1944
Collection
Keywords
Publisher
  • Springer  (134)
  • American Chemical Society
  • Elsevier
  • Institute of Physics
  • Wiley-Blackwell  (3)
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    A microscopic investigation of enamel in wombat (Vombatus ursinus) (1985)
    Springer
    Cell & tissue research 242 (1985), S. 349-355 
    Details
    ISSN: 1432-0878
    Keywords: Teeth (Vombatus ursinus) ; Enamel (regions) ; Ultrastructure ; Enamel (mature and developing)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A study of the enamel of continuously growing Vombatus ursinus molars was carried out using the techniques of light microscopy, hardness testing, scanning electron microscopy and transmission electron microscopy. From the erupted end to within 8 mm of the growing end, mature enamel was observed and it was found that between comparable areas there were no significant ultrastructural differences in enamel; however, small (∼12nm diameter), loosely packed needle-like crystals characteristic of developing enamel were observed near the growing end. Mature enamel was found to consist of three optically-translucent regions interleaved with two opaque regions. Opaque enamel was softer than translucent enamel. The opacity and relative softness characteristic of two of the enamel regions was not related to prism pattern or orientation; it was, however, related to the presence of voids (∼28 nm diameter) in these regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214547
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  • 2
    Electronic Resource
    Electronic Resource
    Gonadotropin production and release in female goldfish (Carassius auratus) after administration of pimozide and an LHRH analogue as studied by electron microscopy and radioimmunoassay (1987)
    Springer
    Cell & tissue research 247 (1987), S. 477-482 
    Details
    ISSN: 1432-0878
    Keywords: Gonadotropic cells ; LHRH analogue ; Pimo-zide ; Ultrastructure ; Goldfish ; Carassius auratus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of pimozide and an LHRH-analogue (LHRH-A) on gonadotropic cells of the goldfish pituitary gland were described qualitatively and quantitatively. A scale of four categories was devised to reflect various ultrastructural appearances of the cells. Experimental animals were divided into a control group, a group injected with LHRH-A alone, pimozide alone, and groups receiving these two substances in combination. Fish injected with the single substance were killed 12 h after injection while the groups receiving the combined treatments were killed at 4, 12 and 48 h. Serum levels of gonadotropin measured by radioimmunoassay were used to indicate whether an increase in hormone release had occurred. An immunocytochemical technique, the protein A-gold procedure, assured that the cells studied were gonadotropes. The control group showed variation in the profiles of gonadotropic cells. The single treatment groups showed some increase in secretory inclusions. At 4 h after injection the combined treatment caused a significant increase in hormone granules; at 12 and 48 h there was a gradual decrease in content of secretory products, and an increase in vacuolization. The results indicate that the combined pimozide and LHRH-A treatment stimulated gonadotropin production as well as release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215739
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  • 3
    Electronic Resource
    Electronic Resource
    Study of neuropeptide Y-containing nerve fibers in the human penis (1988)
    Springer
    Cell & tissue research 254 (1988), S. 69-74 
    Details
    ISSN: 1432-0878
    Keywords: Neuropeptide Y (NPY) ; Penis erection ; Perivascular nerve fibers immunocytochemistry ; Ultrastructure ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neuropeptide Y 1–36 (IR-NPY) immunoreactive nerve-fiber processes have been observed in tunicae of veins and arteries and in smooth muscles of the human penis taken at autopsy or during surgery by use of light-and electron-microscopic immunohistochemical techniques. Numerous IR-NPY nerve fibers were mostly concentrated in the inner part of the adventitia close to the media of the arterial and venous vessels and among the intracavernous smooth muscle cells. IR-NPY nerve fibers were less abundant in veins than in arteries. Positive somata were not observed in the penises. At the ultrastructural level, IR-NPY were localized exclusively in large, dense granules of nerve terminals by means of the postembedding immunogold technique. In the deep dorsal vein, IR-NPY nerve fibers were also located in the media formed by an outer circular and an inner longitudinal layer. In the intracavernous and dorsal arteries, they showed the highest density in the inner part of the adventitia. In the corpora cavernosa and in the corpus spongiosum, IR-NPY nerve processes were intermingled between the smooth-muscle fibers around the sinusoid spaces. IR-NPY nerve fibers were present in the cavernous nerves close to the central arteries. The urethra did not show any IR-NPY-positive nerve fibers. This peculiar distribution of IR-NPY nerve fibers suggested that they could participate in regulating arterial and venous blood flow and intracavernous smooth-muscle tone. NPY may therefore be of importance in some of the mechanisms of penile erection especially during detumescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220018
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  • 4
    Electronic Resource
    Electronic Resource
    Bioanalysis of fluorouracil applying liquid chromatography and valve switching (1988)
    Springer
    Chromatographia 25 (1988), S. 806-810 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ion-pair chromatography ; Valve switching ; Fluorouracil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Fluorouracil has been analyzed with a reversed-phase ion-pair liquid chromatographic system applying PRP-1 as the stationary phase. A valve switching technique was applied for the separation of the analyte and some other chemotherapeutic agents. The described procedure gives a rapid screening for the analyte in urine and plasma samples without interferences from late eluting solutes. Biological samples are pretreated by means of a liquid-liquid extraction resulting in a recovery of about 52% for plasma and urine with minimum detectable amounts of about 5ng/ml. The sensitivity and the selectivity of the procedure allows the monitoring of fluorouracil in body fluids and subsequent pharmaco-kinetic studies of the fluoropyrimidine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02262089
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of brief vascular perfusion on the ultrastructure and activity of mitochondria isolated from the rat heart (1985)
    Springer
    Cell & tissue research 240 (1985), S. 247-249 
    Details
    ISSN: 1432-0878
    Keywords: Heart ; Mitochondria ; Perfusion ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mitochondria isolated from heart tissue after a 1-min perfusion with Hanks medium were found to have significantly lower rates of State-3 respiration and respiratory control ratios compared to mitochondria isolated from non-perfused hearts. Examination of the mitochondrial preparations by electron microscopy revealed that a large proportion of the mitochondria isolated from perfused heart tissue were swollen and broken compared to mitochondria from non-perfused hearts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00217581
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  • 6
    Electronic Resource
    Electronic Resource
    Quantitative analysis of estradiol-17β-induced changes in the ultrastructure of the liver of the male zebrafish, Brachydanio rerio (1985)
    Springer
    Cell & tissue research 242 (1985), S. 377-382 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Hepatocytes ; Ultrastructure ; Estradiol ; Vitellogenesis ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Hepatocytes of male zebrafish, Brachydanio rerio, were studied by means of light- and electron-microscopy, following a period of maximally 16 days of in-vivo treatment with estradiol-17β. The responsiveness of the male hepatocytes to this female sex steroid was investigated by use of morphometric methods. The results of this investigation show that the responsiveness was most obvious between 2 and 16 days, as revealed by an increase in cell size, accompanied by a proliferation of the granular endoplasmic reticulum and the Golgi complex. In addition, accumulations of glycogen granules, which are characteristic of hepatocytes in untreated males, had disappeared and lipid droplets had accumulated. These experimentally induced changes in the morphology of the male hepatocyte closely resemble those described for the female hepatocyte during the sexual cycle. It is concluded that the hepatocytes of male zebrafish can be stimulated by estradiol-17β to produce vitellogenin and that in female zebrafish this steroid is a key sex hormone responsible for vitellogenin production by the liver during the natural sexual cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214551
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  • 7
    Electronic Resource
    Electronic Resource
    Glomerular ultrastructure of the trout, Salmo gairdneri: Effects of angiotensin II and adaptation to seawater (1987)
    Springer
    Cell & tissue research 249 (1987), S. 437-442 
    Details
    ISSN: 1432-0878
    Keywords: Angiotensin II ; Glomerulus ; Salmo gairdneri ; Seawater-adaptation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of angiotensin infusion on the glomerular ultrastructure of freshwater- and seawater-adapted rainbow trout, Salmo gairdneri, has been examined by scanning and transmission electron microscopy. Adaptation of trout to seawater resulted in epithelial podocyte flattening, primary process broadening and apparent loss of foot processes in almost all glomeruli, features which were uncommon in freshwater-adapted trout. Similar changes were induced by infusion of freshwater-adapted animals with angiotensin, suggesting that the renin-angiotensin system plays a role in the modification of glomerular epithelial ultrastructure. Adaptation of trout to seawater also reduced glomerular diameter, but infusion of freshwater-adapted animals with angiotensin did not mirror this effect. Infusion of angiotensin into seawater-adapted animals increased the overall thickness of glomerular basement membrane by increasing the lamina rara interna and lamina densa. This did not occur when freshwater-adapted fish were either infused with angiotensin or adapted to seawater. These findings suggest that other humoral systems are involved in the control of glomerular diameter and basement membrane thickness as part of an integrated response to increased environmental salinity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215528
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  • 8
    Electronic Resource
    Electronic Resource
    Liquid chromatographic capacity factor as an indicator of lipophilicity in a series of betablocker drugs (1986)
    Springer
    Chromatographia 22 (1986), S. 48-50 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Betablocker drugs ; Lipophilicity ; Capacity factors ; Distribution coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The reverse-phase, high-pressure liquid chromatographic capacity factors (k') of a series of 18 betablocking agents were determined in order to establish correlations with their distribution coefficient values (D), obtained from four bibliographic sources. Under the three pH conditions studied (3.5, 6.2 and 7.5) statistically significant linear correlations (p〈 0.002) between log k' and log D values were obtained for the four series studied. In this series it is concluded that the k' factor is a useful and reliable relative lipophilicity parameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02257296
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  • 9
    Electronic Resource
    Electronic Resource
    Invetigations into the use of an auxiliary metal ion for indirect amperometri detection (1988)
    Springer
    Chromatographia 25 (1988), S. 162-166 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ion chromatography ; Analysis of metal ions ; Post column derivatisation ; Amperometric detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A method for determination of metal ions subsequent to separation by ion chromatography has previously been developed. The method is based on Indirect Amperometric Detection whereby the decrease in the oxidation current, due to a dithiocarbamate ligand added post column, is monitored. Upon elution from the chromatographic column the metal ions are complexed by the ligand. As the complexes formed are electroinactive at the applied potential, the background current decreases according to the metal ion concentration. The method developed in this work involves addition to the reagent of Zn(II) as a auxiliary metal ion to displace the analyte metals from the chromatographic eluent ligand complexes after separation. Sodium bis(2-hydroxy-ethyl) dithiocarbamate was used as the postcolumn derivatising reagent. The addition of Zn(II) to the reagent causes some unforeseen behaviour in the chromatographic system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02316437
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  • 10
    Electronic Resource
    Electronic Resource
    Crocheted PTFE reactors for post-column photochemistry in HPLC (1986)
    Springer
    Chromatographia 22 (1986), S. 231-234 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Post-column reactor ; Crosheted PTFE construction ; Photochemical reaction detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Construction of crocheted photochemical reactors for use in high performance liquid chromatography is described. The design of these reactors is optimal for low intensity light sources because of their cylindrical configuration. Band broadening is minimized by the use of tightly crocheted narrow bore tubing and fittings modified to reduce dead volume. These reactors are suitable for most post-column photochemical reactions with the additional possibility of serving as mixing/delay coils, deoxygenation devices for electrochemical detectors, and as low dispersion detector cells for chemiluminesckent reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02268764
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