ISSN:
0739-4462
Keywords:
hybridoma
;
ELISA
;
immunoblotting
;
apolipoproteins
;
locust
;
Chemistry
;
Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Spleen lymphocytes from mice immunized with locust native low-density lipophorin A+ (LDLp) were fused with nonproducing myeloma cells, strain Sp 2/0. Hybridomas that were isolated from the fused cells produced antibodies specific for LDLp and the high-density lipophorin Ayellow (HDLp).Monoclonal strains were generated through cloning by limiting dilution from those hybridomas synthesizing antibodies specific for apolipophorins (apoLp)-I, -II, and -III of LDLp. Additionally, a hybridoma strain that was obtained after fusion of lymphocytes from mice immunized with apoLp-III produced antibodies that bind to apoLp-III and native LDLp.Some features of LDLp and HDLp were studied using these antibodies. It could be demonstrated that apoLp-I and apoLp-II are not immunochemically identical and are exposed in the native particle of both LDLp and HDLp. It was also shown that in both lipophorins apoLp-II is less exposed than apoLp-I, whereas in LDLp apoLp-III is mainly exposed; some apoLp-III could also be detected in HDLp.Tween-20, a nonionic detergent, appears to affect the binding of anti-apoLp-I, -II, and -III to both LDLp and HDLp. The monoclonal antibodies specific for locust apolipophorins do not bind to the respective apoproteins of lipophorins from other insects.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/arch.940060204
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