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  • Datura  (2)
  • Rat (Sprague-Dawley)  (2)
  • 1985-1989  (4)
  • 1950-1954
  • 1930-1934
Collection
Keywords
Publisher
Years
  • 1985-1989  (4)
  • 1950-1954
  • 1930-1934
Year
  • 1
    Electronic Resource
    Electronic Resource
    Nucleotide pools in suspension-cultured cells of Datura innoxia (1985)
    Springer
    Planta 166 (1985), S. 446-451 
    Details
    ISSN: 1432-2048
    Keywords: Datura ; Macromolecular synthesis ; Nucleotide pool ; Nutrient uptake ; Suspension culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A general picture of the metabolic events which govern to growth behaviour of a batch culture of suspended dedifferentiated cells of Datura innoxia is obtained by following both the uptake and accumulation of the medium phosphate and sucrose by the cells, and the synthesis of RNA, protein and starch. The results are compared with the changes in the nucleotide pools described in the preceding paper. The sequence of formation and the regulatory dependencies of cellular pools of phosphate, sucrose, nucleotides and RNA in the production of proteins, starch and cell mass, and in the control of proliferation and cell growth are discussed. Furthermore, the importance of the maintenance pools for metabolic survival during starvation is emphasized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391268
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide pools in suspension-cultured cells of Datura innoxia (1985)
    Springer
    Planta 166 (1985), S. 439-445 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture (nucleotides) ; Datura ; Energy charge ; Nucleoside diphosphate sugar ; Nucleotide pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The concentrations of 17 nucleotides and three nucleosides have been determined in a batch suspension culture of Datura innoxia using a new procedure for extraction, purification and high-performance liquid chromatography separation of these compounds. The nucleotide pools change appreciably in the different phases of growth. These changes indicate the preparation for and initiation of cell proliferation, and reflect metabolic events during cell division, cell elongation and starvation. The main components of the nucleotide pool are uracil nucleotides, with uridine 5′-diphosphate sugars as the predominant fraction, and the adenine nucleotides. Although their concentrations vary by a factor of more than 6 the ratio of the uracil to adenine nucleotides is kept fairly constant during growth. The energy charge is maintained at a rather high value. The correlation of these events with nutrient uptake and macromolecular synthesis by the batch culture is presented in the following paper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391267
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  • 3
    Electronic Resource
    Electronic Resource
    Immature rat epididymal epithelial cells grown in static primary monolayer culture on permeable supports (1989)
    Springer
    Cell & tissue research 256 (1989), S. 567-572 
    Details
    ISSN: 1432-0878
    Keywords: Epididymis ; Monolayer culture ; Directed secretion ; Phosphatases ; Glucosaminidase ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A cell culture system is characterised for monolayers of immature rat epididymal epithelial cells grown on permeable supports. Cover of the filters was achieved by days 4–5 and was maintained for 9–12 days. The secretion of acid phosphatase (ACP), alkaline phosphatase (AKP) and N-acetylglucosaminidase (NAG) into apical and basal compartments of culture chambers was monitored with time in culture for cells from the proximal and distal epididymis of 37-day-old animals. There was independent secretion of the three enzymes: secretion of NAG and AKP was mainly apical, that of ACP basal; daily secretion of ACP and AKP was constant throughout culture, that of NAG declined; there was greater secretion of NAG and AKP by cells from the proximal than the distal region. The initial high apical secretion of NAG is thought to reflect loss of enzyme from unattached cells, whereas the later AKP secretion is truly directional. Secretion was not influenced by the enzymes used in cell preparation. The cytotoxic agent Thimerosal inhibited secretion of all enzymes when placed beneath the cultures, indicating that secretion depended on viable cells, but initially stimulated release of AKP when applied above the cells possibly reflecting release from the cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225605
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  • 4
    Electronic Resource
    Electronic Resource
    Immature rat epididymal epithelial cells grown in static primary monolayer culture on permeable supports (1989)
    Springer
    Cell & tissue research 256 (1989), S. 573-580 
    Details
    ISSN: 1432-0878
    Keywords: Epididymis ; Epithelium ; Monolayer culture ; Histochemistry ; Electron microscopy ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary N-acetylglucosaminidase (NAG), acid phosphatase (ACP) and alkaline phosphatase (AKP) were localised histochemically in fixed cells from the 37-day-old rat epididymis grown in static monolayer culture for 2–8 days. ACP and NAG were cytosolic enzymes found in perinuclear positions, whereas staining of AKP was consistent with a membranous position. These enzymes were also examined in frozen tissue sections of the epididymis, from rats of the equivalent age, where NAG had intense activity in both supra- and infra-nuclear cytoplasm and ACP was more active apically. For the first time AKP was localised along basolateral membranes of the epithelium and in the lumen of the mid-caput region. The monolayer in culture was of principal cells only and they maintained their polarity and ultrastructural characteristics, but the height of the cells was reduced compared to that obtained in situ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225606
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