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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Mathematical programming 42 (1988), S. 327-345 
    ISSN: 1436-4646
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract Most large-scale optimization problems exhibit structures that allow the possibility of attack via algorithms that exhibit a high level of parallelism. The emphasis of this paper is the development of parallel optimization algorithms for a class of convex, block-structured problems. Computational experience is cited for some large-scale problems arising from traffic assignment applications. The algorithms considered here have the property that they allow such problems to be decomposed into a set of smaller optimization problems at each major iteration. These smaller problems correspond to linear single-commodity networks in the traffic assignment case, and they may be solved in parallel. Results are given for the distributed solution of such problems on the CRYSTAL multicomputer.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Annals of operations research 14 (1988), S. 125-145 
    ISSN: 1572-9338
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Economics
    Notes: Abstract This paper presents an application of parallel computing techniques to the solution of an important class of planning problems known as generalized networks. Three parallel primal simplex variants for solving generalized network problems are presented. Data structures used in a sequential generalized network code are briefly discussed and their extension to a parallel implementation of one of the primal simplex variants is given. Computational testing of the sequential and parallel codes, both written in Fortran, was done on the CRYSTAL multicomputer at the University of Wisconsin, and the computational results are presented. Maximum efficiency occurred for multiperiod generalized network problems where a speedup approximately linear in the number of processors was achieved.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 39 (1986), S. 257-268 
    ISSN: 1432-0630
    Keywords: 42.10 ; 42.20 ; 78.20
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Measurements of the reflectance and transmittance in the infrared part of the spectrum (400...4000cm−1) are carried out on oxidized Si wafers. Intrinsic dielectric properties of the oxide layers prepared by various methods are derived from experimental data. The dielectric functions of the oxides can be fitted by oscillator models. The related model parameters are compared with those of other SiO2 samples, as crystals and glasses. Optical arrangements to detect and characterize layers of thicknesses down to 3 nm are discussed. In particular, it is shown that experiments with polarized light at oblique incidence up to 80° are a powerful tool to characterize those layers. Experiments are reported to determine, in addition, extrinsic properties such as B and P atoms in the oxide layer or defects due to an ion implantation treatment. Conditions are discussed which should be applied to obtain the best quantitative analysis of the defect concentration.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 166 (1985), S. 446-451 
    ISSN: 1432-2048
    Keywords: Datura ; Macromolecular synthesis ; Nucleotide pool ; Nutrient uptake ; Suspension culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A general picture of the metabolic events which govern to growth behaviour of a batch culture of suspended dedifferentiated cells of Datura innoxia is obtained by following both the uptake and accumulation of the medium phosphate and sucrose by the cells, and the synthesis of RNA, protein and starch. The results are compared with the changes in the nucleotide pools described in the preceding paper. The sequence of formation and the regulatory dependencies of cellular pools of phosphate, sucrose, nucleotides and RNA in the production of proteins, starch and cell mass, and in the control of proliferation and cell growth are discussed. Furthermore, the importance of the maintenance pools for metabolic survival during starvation is emphasized.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 166 (1985), S. 439-445 
    ISSN: 1432-2048
    Keywords: Cell culture (nucleotides) ; Datura ; Energy charge ; Nucleoside diphosphate sugar ; Nucleotide pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The concentrations of 17 nucleotides and three nucleosides have been determined in a batch suspension culture of Datura innoxia using a new procedure for extraction, purification and high-performance liquid chromatography separation of these compounds. The nucleotide pools change appreciably in the different phases of growth. These changes indicate the preparation for and initiation of cell proliferation, and reflect metabolic events during cell division, cell elongation and starvation. The main components of the nucleotide pool are uracil nucleotides, with uridine 5′-diphosphate sugars as the predominant fraction, and the adenine nucleotides. Although their concentrations vary by a factor of more than 6 the ratio of the uracil to adenine nucleotides is kept fairly constant during growth. The energy charge is maintained at a rather high value. The correlation of these events with nutrient uptake and macromolecular synthesis by the batch culture is presented in the following paper.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 256 (1989), S. 567-572 
    ISSN: 1432-0878
    Keywords: Epididymis ; Monolayer culture ; Directed secretion ; Phosphatases ; Glucosaminidase ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A cell culture system is characterised for monolayers of immature rat epididymal epithelial cells grown on permeable supports. Cover of the filters was achieved by days 4–5 and was maintained for 9–12 days. The secretion of acid phosphatase (ACP), alkaline phosphatase (AKP) and N-acetylglucosaminidase (NAG) into apical and basal compartments of culture chambers was monitored with time in culture for cells from the proximal and distal epididymis of 37-day-old animals. There was independent secretion of the three enzymes: secretion of NAG and AKP was mainly apical, that of ACP basal; daily secretion of ACP and AKP was constant throughout culture, that of NAG declined; there was greater secretion of NAG and AKP by cells from the proximal than the distal region. The initial high apical secretion of NAG is thought to reflect loss of enzyme from unattached cells, whereas the later AKP secretion is truly directional. Secretion was not influenced by the enzymes used in cell preparation. The cytotoxic agent Thimerosal inhibited secretion of all enzymes when placed beneath the cultures, indicating that secretion depended on viable cells, but initially stimulated release of AKP when applied above the cells possibly reflecting release from the cell membrane.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 256 (1989), S. 573-580 
    ISSN: 1432-0878
    Keywords: Epididymis ; Epithelium ; Monolayer culture ; Histochemistry ; Electron microscopy ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary N-acetylglucosaminidase (NAG), acid phosphatase (ACP) and alkaline phosphatase (AKP) were localised histochemically in fixed cells from the 37-day-old rat epididymis grown in static monolayer culture for 2–8 days. ACP and NAG were cytosolic enzymes found in perinuclear positions, whereas staining of AKP was consistent with a membranous position. These enzymes were also examined in frozen tissue sections of the epididymis, from rats of the equivalent age, where NAG had intense activity in both supra- and infra-nuclear cytoplasm and ACP was more active apically. For the first time AKP was localised along basolateral membranes of the epithelium and in the lumen of the mid-caput region. The monolayer in culture was of principal cells only and they maintained their polarity and ultrastructural characteristics, but the height of the cells was reduced compared to that obtained in situ.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 123 (1942), S. 71-77 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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  • 9
    ISSN: 1615-6102
    Keywords: Electrogenic proton pump ; Living cell fragments ; Membrane potential ; Membrane surface coat ; Physarum polycephalum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Small, spherical cell fragments derived from macroplasmodia of the acellular slime moldPhysarum polycephalum by incubation in a 15 mM caffeine solution were investigated with morphological and electrophysiological techniques. Analysis of cell surface composition with the fluorescence microscope and different RITC-conjugated lectins revealed strong binding of ConA and RCA-I, weak binding of PEA, DBA and WGA and no binding of UEA-I. In addition, binding sites for external calcium ions were detected by chlorotetracycline-fluorescence. Electron microscopical staining with ruthenium red, iron or lanthanum delivered evidence for localization of lectin and calcium binding sites in a thin mucous layer on the cell surface. Electrical recordings by means of intracellular microelectrodes yielded an average membrane potential (MP) of −113 mV. Spontaneous depolarizations of the MP, with amplitudes between 10 and 80 mV and a duration of 20–30 s, failed to show a correlation with contractile activity. The ionic nature of MP was studied by varying the composition of the perfusing medium. The MP was not much affected by changes in external [Ca2+], [K+], or [Na+] but was sensitive to changes in [Cl−] or [H+], with a linear dependence on pH0 in the range between 7 and 5. Metabolic inhibition by potassium cyanide or low temperature (11°C) as well as application of the protonophore CCCP caused a depolarization of the MP. The results strongly support the hypothesis that the MP inPhysarum cell fragments is mainly generated by an electrogenic H+-extrusion mechanism.
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  • 10
    ISSN: 1573-0867
    Keywords: Durum ; grain yield ; irrigation ; modoc ; nitrogen ; response surface model ; seeding rate ; Triticum turgidum ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interactive effects of nitrogen (N) rates, seeding (S) rates and irrigation frequencies on grain yield and yield components of durum wheat were studied for four years under field conditions at Tulelake, California. Each year the experiment was conducted using a split-plot design with 4 irrigation frequencies as main plots and combinations of 5 N-rates (0 to 360 kg/ha) and 5 S-rates (50 to 250 kg/ha) as subplot treatments replicated 4 times. A quadratic response surface model (RSM) was used to study the effects of these treatments on grain yield and yield components (tillers/area, kernel number/spike, kernel weight/spike and 100-seed weight). The RSM was very effective for analysis and data reduction for estimating the optimum combinations of N and S for maximizing the grain yield and yield components. The N utilization and uptake efficiency increased with each irrigation treatment and peaked at irrigation treatment C. Both N and uptake utilization efficiency decreased with each increment of N-rate. In most cases, the effect of irrigation was independent of N and S. One irrigation at tillering increased grain yield and yield components significantly over only a preplant irrigation. The response of additional irrigations were comparatively small and significant only in some cases. Both N and S had significant effects on grain yield and yield components, however, the response of N was larger than that of S. With increasing N-rate, grain yield and tiller number increased with the expected peak beyond 360kg N ha−1 but the increments beyond 180 kg N ha−1 were of progressively smaller magnitude. The kernel number and kernel weight per spike also increased with N-rate giving a peak between 270 and 360 kg N ha−1. With increasing S grain yield and tiller number/area increased while kernel number and kernel weight per spike decreased progressively. It was impossible to maximize yield and yield components at a given combination of N, S, and irrigation. According to the model, grain yield and tiller number were maximized at the highest level of N and S, while kernel number and kernel weight/spike were maximized at the lowest S (50 kg ha−1) and about 314 kg N ha−1 under adequate water supply. On the basis of the findings of this study and output of the model, 180–360 kg N ha−1, 150–250 kg S ha−1 and two post-sowing irrigations (at tillering and at boot stage) in addition to a preplant irrigation was recommended for optimum yield. An additional irrigation might be required depending on the weather conditions during the grain filling period.
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