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  • Blood analysis  (1)
  • 1985-1989  (1)
  • 1955-1959
  • 1
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Nitrogen-specific detector ; Flash methylation ; Barbital and pentobarbital ; Blood analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A sensitive and simplified method is described for the determination of barbital (B) and pentobarbital (PB) in blood using gas chromatography with a nitrogen-specific flame ionization detector. After a simple one-step extraction, B and PB were reconstituted in trimethylanilinium hydroxide and introduced directly into the gas chromatograph. Lower limits of detection was 0.14 μg/ml for B and PB in either water or blood solutions. The recoveries for B from water and blood were 90 and 88%, respectively; for PB they were essentially complete. The average between-run and within-run coefficients of variation for B from water and blood were lower than 7.8%, whereas those of PB were lower than 3.0%. The scale and sensitivity of detection for B and PB were found to be suitable for use in pharmacokinetic studies in the rat.
    Type of Medium: Electronic Resource
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