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  • DNA sequence  (2)
  • Springer  (2)
  • American Geophysical Union
  • National Academy of Sciences
  • 1985-1989  (2)
  • 1955-1959
Collection
Keywords
Publisher
  • Springer  (2)
  • American Geophysical Union
  • National Academy of Sciences
Years
  • 1985-1989  (2)
  • 1955-1959
Year
  • 1
    Electronic Resource
    Electronic Resource
    The cloning and characterization of a RAS gene fromSchizosaccharomyces pombe (1986)
    Springer
    Journal of molecular evolution 23 (1986), S. 41-51 
    Details
    ISSN: 1432-1432
    Keywords: RAS oncogene ; Cloning ; DNA sequence ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned and determined the complete nucleotide sequence of a RAS gene from the yeastSchizosaccharomyces pombe (SP-RAS). The putative RAS protein of 214 amino acids is encoded by two noncontiguous reading frames separated by an intron of 86 bp. The SP-RAS gene product shares extensive homology with the proteins of theSaccharomyces cerevisiae (SC),Dictyostelium, Drosophila, and human RAS genes in its N-terminal region but not in its C-terminal region. The extended C-terminal regions found in the SC-RAS genes have no counterpart in the SP-RAS gene. Thus the RAS genes of these two yeasts are structurally quite distinct. The SP-RAS sequence was expressed in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100997
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence analysis of three fragments of maize nuclear DNA which replicate autonomously in yeast (1988)
    Springer
    Plant molecular biology 11 (1988), S. 173-182 
    Details
    ISSN: 1573-5028
    Keywords: ARS ; maize ; DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There maize nuclear DNA fragments were isolated on the basis of their ability to confer replication on chimeric plasmids in yeast. These Eco RI fragments of 2.5, 2.8 and 5.5 kb are repeated elements within the maize genome. The 2.5 and 2.8 kb fragments represent a family of elements repeated 11 000 times in the maize haploid genome, while the 5.5 kb fragment is part of another family of 28 000 elements. These fragments were subcloned to further define the unique region of ARS activity. The sequence of each 550–650 bp ARS subclone is reported here, and compared to the flanking regions which do not show ARS activity. The ARS elements are 65–70% A+T as compared to 50–55% for the maize genome as a whole. There is approximately 15% sequence divergence, as well as variation of ARS efficiency, among family members. ARS subclones contain the proposed yeast consensus sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015669
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    Paper (German National Licenses)
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