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  • Chemistry  (8)
  • Evolution  (2)
  • 5S RNA  (1)
  • 1985-1989  (10)
  • 1960-1964  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Unusual sequences, homologous to 5S RNA, in ribosomal DNA repeats of the nematodeMeloidogyne arenaria (1988)
    Springer
    Journal of molecular evolution 27 (1988), S. 222-227 
    Details
    ISSN: 1432-1432
    Keywords: Ribosomal DNA ; 5S RNA ; Nematode ; Molecular phylogeny ; Progenote ; Gene families
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary There are sequences homologous to 5S ribosomal RNA in the ribosomal DNA (rDNA) repeats of the plant-parasitic nematodeMeloidogyne arenaria. This is surprising, because in all other higher eukaryotes studied to date, the genes for 5S RNA are unlinked to and distinct from a tandem rDNA repeat containing the genes for 18S, 5.8S, and 28S ribosomal RNA. Previously, only prokaryotes and certain “lower eukaryotes” (protozoa and fungi) had been found to have both the larger rRNAs and 5S rRNA represented within a single DNA repeat. This has raised questions on the organization of these repeats in the earliest cell (progenote), and on subsequent evolutionary relationships between pro- and eukaryotes. Evidence is presented for rearrangements and deletions withinMeloidogyne rDNA. The unusual life cycles (different levels of ploidy, reproduction by meiotic and mitotic parthenogenesis) of members of this genus might allow rapid fixation of any variants with introduced 5S RNA sequences. The 5S RNA sequences inMeloidogyne rDNA may not be expressed, but their presence raises important questions as to the evolutionary origins and stability of repeat gene families.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100078
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  • 2
    Electronic Resource
    Electronic Resource
    Histone genes in three sea star species: Cluster arrangement, transcriptional polarity, and analyses of the flanking regions of H3 and H4 genes (1988)
    Springer
    Journal of molecular evolution 27 (1988), S. 36-44 
    Details
    ISSN: 1432-1432
    Keywords: Histone genes ; Sea stars ; Echinoderms ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The arrangement of core histone genes and their transcriptional polarity has been determined for three species of sea stars (Pisaster ochraceus, P. brevispinus, andDermasterias imbricata) representing two orders which diverged over 500 million years ago. Each species has approximately 500 core histone cluster repeats per haploid genome. The close phylogenetic relationship between thePisaster species is evident from the correspondence of restriction sites in the repeat element, identical arrangement of core histones, and high degree of sequence homology in both the coding and spacer regions of the H3 gene. TheDermasterias repeat has the same gene order and transcriptional polarity of core histones, but its restriction map is significantly different. Moreover, theDermasterias H3 gene has the same amino acid sequence, but in comparison toPisaster nucleotide sequences, shows a high level of silent substitutions. Analyses of the nucleotide sequence of the 5′ and 3′ regions surrounding the H3 gene from each species demonstrate the presence of appropriately spaced consensus and processing signal segments. The 3′ spacer segment of theDermasterias H4 gene contains an unusual, threefold tandemly repeated, 21-nucleotide, AT-rich sequence. No similar sequence is seen in theP. brevispinus H4 3′ region, but these two species show a striking regularity of distribution of five different homologous oligomers in the 3′ spacer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099728
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  • 3
    Electronic Resource
    Electronic Resource
    Organization and unusual expression of histone genes in the sea starPisaster ochraceus (1987)
    Springer
    Journal of molecular evolution 25 (1987), S. 29-36 
    Details
    ISSN: 1432-1432
    Keywords: Histone genes ; Echinoderms ; Maternal mRNA ; Development ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization and maternal expression of histone genes differ greatly between the two echinoderm classes represented by the sea stars and sea urchins. We have isolated and characterized a 5.3-kb genomic DNA fragment encoding core histone genes in the sea starPisaster ochraceus. The majority of histone genes are organized as tandem repeats of the 5.3-kb fragment, which is present in as many as 700 copies per haploid genome. The identity, precise location, and transcriptional polarity of individual core histone genes within the repeat were determined by DNA sequence analyses. The gene order in the sea star (H2B, H2A, H4, H3) is different from that in sea urchins (H2B, H3, H2A, H4). What is remarkable is the low level of maternal histone mRNA inP. ochraceus eggs relative to that in sea urchins. This observation supports a recent suggestion that major changes in mode of gene expression, rather than in gene organization or copy number, can be correlated with major events in echinoderm evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02100038
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  • 4
    Electronic Resource
    Electronic Resource
    Positive ion fast atom bombardment mass spectrometry of reductively pyridylaminated maltooligosaccharides and its application to α-amylase assay (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 233-237 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Positive ion fast atom bombardment mass spectra of maltooligosaccharides reductively aminated with 2-aminopyridine (Gn-AP) contain abundant [M + H]+ ions. Determination of G2-AP and G3-AP produced from G5-AP by the action of α-amylases, based on the abundance of their [M + H]+ ions relative to that of cellobiose reductively aminated with 2-amino-6-methylpyridine as the internal standard, allowed rapid and reproducible assay of these enzymes. It was advantageous for clinical investigation that the proportion of pancreatic and salivary α-amylase activities could be determined.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200150408
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  • 5
    Electronic Resource
    Electronic Resource
    Temperature dependence of nuclear magnetic resonance absorption in coal (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 6 (1962), S. 94-97 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The proton magnetic resonance in the vitrain of Yūbari caking coal and its residue of pyridine extraction has been studied over the range 90 to 423°K. At lower temperature the absorption line consists of a broad component. At higher temperature this component begins to decrease rapidly in the line width and resolves into two components. This rapid narrowing of the absorption line width suggests a glass transition attributed to the molecular motion in coal. The variation of the second moment of the absorption line with temperature reflects the line width variation. From these results some information on the molecular motion and arrangement in coal have been obtained.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1962.070061910
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on the constituents of marine opisthobranchia, III. Isolation and structure of two 5,8α-epidioxysterols and a cholesteryl ester mixture from the albumen gland of Aplysia juliana (1988)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1988 (1988), S. 589-592 
    Details
    ISSN: 0170-2041
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Studien über Inhaltsstoffe von Opisthobranchia, III. - Isolierung und Struktur von zwei 5,8α-Epidioxysterolen und von einem Cholesterylester-Gemisch aus der Albumen-Drüse des See-Hasen Aplysia julianaAus der Albumen-Drüse des See-Hasen Aplysia juliana, der in der Provinz Kumamoto gesammelt worden war, wurden das Cholesterylester-Gemisch 1 und die zwei 5,8α-Epidoxisterole 5 und 8 isoliert. Säurekatalysierte Methanolyse von 1 ergab die Methylester von vier Fettsäuren [Palmitat (Hauptbestandteil), Stearat, Margarat und ein Isomeres von Margarat] und Cholesterin. Auf Grund synthetischer und spektroskopischer Untersuchungen wurden die Strukturen von 5 und 8 als 5,8α-Epidioxy-5α-cholest-6-en-3β-ol und 5,8α-Epidioxy-5α-cholesta-6,9(11)-dien-3β-ol festgestellt.
    Notes: From the albumen glands of the sea hare Aplysia juliana, collected in Kumamoto Prefecture, a mixture of cholesteryl esters (1) and two 5,8α-epidioxysterols 5 and 8 have been isolated. Acid-catalyzed methanolysis of 1 afforded four methyl esters of fatty acids [palmitate (major component), stearate, margarate, and an isomer of margarate] and cholesterol. 5 and 8 were identified by spectroscopic and synthetical evidence to be 5,8α-epidioxy-5α-cholest-6-en-3β-ol and 5,8α-epidioxy-5α-cholesta-6,9(11)-dien-3β-ol, respectively.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.198819880616
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  • 7
    Electronic Resource
    Electronic Resource
    Biologically active glycosides from asteroidea, XV. Asymmetric synthesis of phytosphingosine and phytosphingosine anhydro base: Assignment of the absolute stereochemistry (1988)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1988 (1988), S. 619-625 
    Details
    ISSN: 0170-2041
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Biologisch aktive Glycoside aus Asteroidea, XV.  -  Asymmetrische Synthese von Phytosphingosin und Phytosphingosinanhydrobase: Ermittlung der absoluten StereochemieDie erste asymmetrische Synthese eines Phytosphingosins, (2S,3S,4R)-2-Amino-1,3,4-hexadecantriol (2), wurde mit Hilfe von kinetischer Racemattrennung und asymmetrischer Epoxidation durchgeführt. Die absolute Stereochemie von Phytosphingosin aus Seesternen (Acanthaster planci und Asterina pectinifera) stimmt mit der von 2 überein. Die Phytosphingosinanhydrobase 19 wurde ebenfalls durch asymmetrische Synthese dargestellt und die Struktur mit Hilfe von 2D-COSY-, NOESY- und Differenz-NOE-Experimenten ermittelt.
    Notes: The first asymmetric synthesis of a phytosphingosine, (2S,3S,4R)-2-amino-1,3,4-hexadecanetriol (2), was accomplished by kinetic resolution and asymmetric epoxidation. The absolute stereochemistry of the phytosphingosine from starfishes (Acanthaster planci and Asterina pectinifera) was established to be the same as for 2. The phytosphingosine anhydro base 19 was also asymmetrically synthesized, and the structure was determined on the basis of NMR analyses involving 2D-COSY, NOESY, and difference NOE experiments.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.198819880702
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  • 8
    Electronic Resource
    Electronic Resource
    Reduktive Oligomerisierung von 1,2-Di-tert-butyl-1,1,2,2-tetrachlordisilan: Das Tetracyclo[3.3.0.02,7.03,6]octasilan- und das Tricyclo[2.2.0.02,5]hexasilan-SystemDer am MIT durchgeführte Teil dieser Arbeit wurde durch die National Science Foundation (USA) gefördert. (1988)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 100 (1988), S. 1793-1795 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19881001228
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  • 9
    Electronic Resource
    Electronic Resource
    Studies of 13C NMR spectra of 13C-enriched cycloartenol biosynthesized from [1-13C]-, [2-13C]-and [1,2-13C2]-acetate. Revised 13C NMR spectral assignments of cycloartenol and cycloartanol and 13C NMR spectral support for the generally accepted skeleton formation mechanism of cycloartenol (1987)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 25 (1987), S. 683-687 
    Details
    ISSN: 0749-1581
    Keywords: 13C-enrichment ; 13C NMR ; spectral assignment ; Cycloartanol ; Cycloartanol ; Skeleton formation mechanism ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Previously proposed 13C NMR spectral assignments of cycloartenol (9,19-cyclolanost-24-en-3β-ol) (1) and cycloartanol (9,19-cyclolanostan-3β-3-ol) (2) were re-examined by means of 13C-enrichment experiments and LIS measurement of 13C chemical shifts. Revised signal assignments are proposed for C-7, C-11, C-16, C-18, C-21 and C-28 of 1 and C-7, C-11, C-12, C-15 and C-16 of 2. Experimental proof for the skeleton formation mechanism of 1 based on 13C NMR spectral findings is presented.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260250806
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  • 10
    Electronic Resource
    Electronic Resource
    Conformations of bryonolic acid and its derivatives in CDCl3 solution by 1H and 13C NMR spectroscopy (1987)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 25 (1987), S. 848-855 
    Details
    ISSN: 0749-1581
    Keywords: Conformation ; D:C-friedoolean-8-enes ; Molecular mechanics calculation ; 1H and 13C NMR ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Comparison of the 1H and 13C chemical shift data of bryonolic acid (D : C-friedoolean-8-en-3β-ol-29-oic acid) and its derivatives (13 species) revealed that the conformational features of these compounds in CDCl3 solution depend on whether the functional groups at C-29 are trigonal, such as COOCH3, or tetrahedral, such as CH2OH. In order to study the conformational features of these two groups of compounds, vicinal coupling constants between the protons on the E rings of the methyl ester of D : C-friedoolean-8-en-29-oic acid, D : C-friedoolean-8-en-29-oic and its acetate were measured. Comparison of the results with the steric geometries to be expected from the molecular mechanics calculation for the E-ring moieties of these derivatives in the S form (D-E rings: boat-boat form) and the F form (D-E rings: chair-chair form) led to the conclusion that the compounds having a trigonal functional group at C-29 mainly adopt the S form, and those having a tetrahedral functional group at C-29 are present as mixtures of the S and F form in equilibrium in the solutions.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260251004
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