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  • Polymer and Materials Science  (30)
  • Cell & Developmental Biology  (19)
  • Wiley-Blackwell  (49)
  • 1985-1989  (42)
  • 1960-1964  (7)
  • 1920-1924
  • 1
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 24 (1986), S. 1161-1171 
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The relationship between chemical structure and low-temperature modulus of ultraviolet(UV)-radiation-cured optical-fiber coating materials has been investigated. The coating materials are low-viscosity liquid compounds consisting of a 1,4-polybutadiene diacrylate oli-gomer, a monomer, and a photoinitiator. Dynamic mechanical test results show that low-temperature modulus and glass transition temperature (Tg) are affected by the monomer structure and monomer concentration in the coating material in cases where the same oligomer is used. With increasing alkyl group chain length of the alkylacrylate monomer, the Tg value is shifted to lower temperatures. Low-temperature modulus increases with increasing monomer concentration in the coating material. Low-temperature excess optical losses of coated fibers have been found to be closely related to low-temperature modulus of the coating.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 32 (1986), S. 4573-4580 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Nylon-3 prepared by hydrogen transfer polymerization of acrylic amide was N-methoxymethylated by paraformaldehyde/methanol in its formic acid solution. This product was soluble in water, and we were able to obtain a transparent membrane by casting of the solution. Absorbance of the NH group on IR spectrum was decreased by methoxymethylation, and from this depression of absorbance a degree of N-methoxymethylation of about 33% was obtained which agreed with the value from elemental analysis. DSC curve of this polymer showed a endothermic peak at 80°C which was assumed to be the Tg and a exothermic peak starting at 180°C which was assumed to be the crosslinking reaction. The degree of crystalinity of this polymer was about 40% which was obtained by deuterium exchange reaction with deuterium oxide. By heating of this polymer membrane up to 190°C, it was changed to an insoluble polymer by the crosslinkage reaction between methoxymethyle and unreacted amide groups. This insoluble polymer membrane was used for the separation of water/alcohol mixture by the pervaporation technique. Through the membrane, water was permeated selectively compared with alcohol and the selective permeation was found to increase by raising the heat treatment temperature.
    Additional Material: 9 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 33 (1987), S. 3019-3036 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The macroscopic order parameter S in the cholesteric liquid crystalline phase of hydroxypropyl cellulose (HPC) and ethyl cellulose (EC) solutions was calculated using refractive index data. Haller's extrapolation approach was used. The S calculated was compared with the S predicted from Doi's theory. The calculated values of S for HPC and EC solutions were in the range of 0.4-0.8 within our experimental range, and agreed with the predicted values of S obtained with Doi's theory. This result suggests that Haller's approach is valid for determining S for liquid crystalline solutions of HPC and EC, despite the scattering of the data at higher temperatures.
    Additional Material: 24 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 20 (1986), S. 879-886 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Iron-containing fluoridated hydroxyapatites with various fluoride contents were synthesized at 80°C and pH 7.4 using FeCl2 · nH2O as a source of iron. The Fe2+ uptake of fluoridated apatites was independent of fluoride concentration in the solution. a-Axis dimensions of Fe-containing apatites decreased with the degree of fluoridation in addition to the decrease related to the substitution of Fe2+ ions. All Fe-containing fluoridated apatites were less well crystallized than Fe-free fluoridated apatites previously reported, although with increasing degree of fluoridation, the crystallinity behavior of the former apatites appeared analogous to that of the latter apatites. In contrast to this inhibited crystallinity behavior, the apparent solubility of Fe-containing fluoridated apatites decreased more than that of Fe-free fluoridated apatites at low fluoride content.
    Additional Material: 6 Ill.
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  • 5
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Mouse macrophages transformed by a temperature-sensitive mutant (tsA640) of simian virus 40 (SV40) were examined by immunofluorescence microscopy for fibronectin expression and actin distribution. Resting cultures of tsA640 transformants incubated at a temperature nonpermissive for SV40 large T antigen (39.0°C) exhibited phagocytic activity and did not exhibit cellular fibronectin and actin cables, like primary cultures of resident macrophages. When the resting cultures were sparsely seeded and shifted down to the permissive temperature of 33.0°C, expression of large T antigen in the nucleus, expression of fibronectin in the cytoplasm, and cellular entry into S phase occurred in that temporal order, followed by actin cable formation, cellular proliferation, and diminishment of phagocytic activity. The expression of T antigen and fibronectin was sensitive to actinomycin D and cycloheximide. The expression of fibronectin was insensitive to inhibitors of DNA synthesis, whereas the expression of actin cables was sensitive. These results suggest that SV40 T antigen leads macrophages to express fibronectin and actin cables, as well as resumption of cell proliferation, and that entry into S phase is not required for fibronectin expression but may be required for actin cable formation.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 133 (1987), S. 95-99 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Platelet-derived growth factor (PDGF) is the major growth factor in serum and a potent mitogen for cells mesenchymal origin. It is a highly basic heterodimeric protein with a molecular mass of -30 kDa and binds to a cell surface receptor with high affinity. The amino acid sequence of PDGF revealed sequence homology to the v-sis gene product of simian sarcoma virus (SSV), a transforming retrovirus. Characterization of cells transformed by SSV has revealed PDGF-related proteins in subcellular organelles and in conditioned media consistent with the autocrine stimulation of cell growth through cell surface receptors and perhaps through an internal autocrine mechanism as the growth factor and its receptor are processed. PDGF is also a potent chemotactic agent for inflammatory and other mesenchymal cells and has been implicated in normal tissue repair processes such as wound healing, as well as in aberrant proliferative processes like atherogenesis.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 122 (1985), S. 210-214 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Four temperature-sensitive mutants of rat 3Y1 fibroblasts belonging to separate complementation groups (3Y1tsD123, 3Y1tsF121, 3Y1tsG125, and 3Y1tsH203) are arrested mainly with a 2C DNA content, when cells proliferating at 33.8°C are shifted up to 39.8°C (Ohno et al., 1984). Zaitsu and Kimura (submitted for publication) showed that 3Y1tsF121 cells synchronized in the early S phase were arrested with a 4C DNA content at 39.8°C. We studied the traverse through the S and G2 phases at 39.8°C in the four ts mutants synchronized at the early S phase and found that 3Y1tsG125 and 3Y1tsH203 cells were arrested with a 4C DNA content as 3Y1tsF121, while 3Y1tsD123 cells went through S and G2 phases and underwent mitosis. When 3Y1tsF121 and 3Y1tsG125 mutants arrested at 39.8°C were shifted down to 33.8°C, a substantial fraction of the cells with a 4C DNA content started, with a certain lag period, DNA synthesis without intervening mitosis and underwent the first mitosis with a lag period similar to that in the cells arrested with a 2C DNA content. The tetraploid cells thus generated had a proliferating ability lower than that of diploid cells.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 125 (1985), S. 223-228 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Metallothionein (MT) synthesis in rabbit kidney-derived RK-13 cells was studied. In response to Cd2+, RK-13 cells synthesized proteins closely similar in chromatographic and electrophoretic behaviors to the liver MTs induced in Cd2+ -injected rabbit. These proteins were specifically immunoprecipitated by anti-mouse liver MT-II serum. The rate of RK-13 thionein (apoprotein of Mt) synthesis rapidly increased after exposure to 1μg/ml of Cd2+, and reached the maximum in 7 h. The dose-response curve for the synthesis was biphasic; a sharp increase up to 0.5 μg/ml Cd2+ and a slower increase at higher concentrations. RK-13 cells retained kidney-specific properties in terms of responsiveness of thionein synthesis to inducers; The MTs were inducible also by Zn2+ and probably by Hg2+, but not by dexamethasone. This system would therefore be a useful model in vitro for studying the regulation of MT synthesis in kidney cells.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 125 (1985), S. 235-242 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We studied the effect of sodium butyrate, a potent G1/G2-arresting agent, on actin distribution in rat 3Y1 fibroblasts in monolayer culture by fluorescence microscopy of cells stained with 7-nitrobenz-2-oxa-1, 3-diazole phallacidine (NBD-Ph). When randomly proliferating cells were arrested mainly in G1 phase with butyrate, a reversible overaccumulation of cellular net protein occurred. In the G1-arrested cells, actin markedly accumulated at the margin of cells, and a network structure of actin stress fibers appeared. When density-arrested cells were replated sparsely and rearrested in the G1, early S, and G2 phases with butyrate or hydroxyurea, the actin network was observed extensively in the cells arrested in the G1 and G2 phases with butyrate. These results agree with our previous results indicating the existence of some physiological similarity between cells in the G1 and G2 phases and suggest that actin distribution somehow depends on the phases of the cell cycle. The actin profiles observed by the NBD-Ph staining were confirmed by transmission electronmicroscopy (TEM) of negatively stained whole cells. TEM further revealed that electron-dense amorphous materials were present at crossing points in the network but rarely present on interconnecting microfilament bundles.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 123 (1985), S. 305-309 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Randomly proliferating 3Y1stD123 cells are arrested in G1 phase within 24 h after a shift up to 39.8°C (temperature arrest), yet the density-arrested cells (prepared at 33.8°C) enter S phase at 39.8°C with serum stimulation, with or without preexposure to 39.8°C for 24 h (Zaitsu and Kimura 1984a). When the density-arrested 3Y1tsD123 cells were preexposed to 39.8°C for 96 h, they lost the ability to enter S phase at 39.8°C by serum stimulation and required a longer lag time to enter S phase at 33.8°C by serum stimulation than did the cells not preexposed to 39.8°C. Simian virus 40 induced cellular DNA synthesis at 39.8°C in the density-arrested 3Y1tsD123 preexposed to 39.8°C for 96 h. In the absence of serum after a shift down to 33.8°C, the temperature-arrested 3Y1stD123 cells entered S phase and then divided once. We postulate from these results that (1) that ts defect in 3Y1tsD123 is involved in a serum-independent process. Once this process is accomplished, its accomplishment is invalidated slowly with preexposure to 39.8°C. This and the serum-dependent processes occur in parallel but not necessarily simultaneously. The accomplishment of both (all) processes is required for the initiation of S phase. The density-arrested 3Y1tsD123 cells have accomplished the serum-independent process related to the ts defect, but have not accomplished serum-dependent processes. In case of the temperature-arrested 3Y1tsD123 cells, the reverse holds true. The lag time for entry into S phase depends on the preparedness for the initiation of DNA synthesis (on the extent of accomplishment of each of all processes required for entry into S phase). (2) To induce cellular DNA synthesis, simian virus 40 stimulates directly the serum-independent process. However, we do not rule out the possibility that simian virus 40 stimulates serum-dependent processes simultaneously.
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