Publication Date:
1986-07-18
Description:
The myb gene is the transforming oncogene of the avian myeloblastosis virus (AMV); its normal cellular homolog, c-myb, is conserved across a broad span of evolution. In humans, c-myb is expressed in malignant hematopoietic cell lines and in primary hematopoietic tumors. Partial complementary DNA clones were generated from blast cells of patients with acute myelogenous leukemia. The sequences of the clones were compared to the c-myb of other species, as well as the v-myb of AMV. In addition, the carboxyl terminal region of human c-myb was placed in an expression vector to obtain protein for the generation of antiserum, which was used to identify the human c-myb gene product. Like v-myb, this protein was found within the nucleus of leukemic cells where it was associated with the nuclear matrix. These studies provide further evidence that c-myb might be involved in human leukemia.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Slamon, D J -- Boone, T C -- Murdock, D C -- Keith, D E -- Press, M F -- Larson, R A -- Souza, L M -- CA36827/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1986 Jul 18;233(4761):347-51.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3014652" target="_blank"〉PubMed〈/a〉
Keywords:
*Aspartate Carbamoyltransferase
;
Avian Leukosis Virus/*genetics
;
Avian Myeloblastosis Virus/*genetics
;
Base Sequence
;
*Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
;
Cell Line
;
Cloning, Molecular
;
DNA/analysis
;
DNA Restriction Enzymes/metabolism
;
*Dihydroorotase
;
Escherichia coli/genetics
;
Hematopoietic Stem Cells/microbiology
;
Humans
;
Leukemia, Myeloid, Acute/*genetics
;
Molecular Weight
;
*Multienzyme Complexes
;
*Oncogenes
;
Proteins/analysis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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