ISSN:
1432-0983
Keywords:
Yest
;
ATPase
;
Galactose promoter
;
Plasma membrane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary In order to probe the physiological role of the yeast plasma membrane ATPase we have replaced the constitutive promoter of its gene by a galactose-dependent promoter. The resulting cells stop growing on glucose medium when the preformed ATPase is diluted to 20% of normal. There is a correlation between ATPase activity and both proton efflux from the cells and amino acid transport. A large proportion of growth-arrested cells appear enlarged and with several buds containing nuclei.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00434664
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