ISSN:
1432-203X
Keywords:
Zea mays L.
;
supersweet corn
;
protoplasts
;
haploids
;
plant regeneration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Plants were regenerated from maize (Zea mays L.) protoplasts isolated from embryogenic cell suspensions. The donor maize suspension cultures were established from friable callus initiated from microspores of a commercial supersweet hybrid (sh2sh2). The frequency of cell colony formation was higher when protoplasts were cultured on feeder layers of maize cells as compared with a liquid thin layer method. It was demonstrated that haploid and dihaploid soil-grown plants can be regenerated from maize protoplasts isolated from haploid cell cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00716662
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