ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Amounts of soluble protein increased three- to four-fold while microsomal protein and polysomal RNA levels decreased by similar factors in cocoa seed extracts at different times during the developmental period of rapid cotyledon growth to fruit ripening. During early development, up to 90% of translatable mRNA was poly A(−). In mature seeds this decreased to about 75%. The radioactive polypeptides synthesized by a. wheat germ in vitro translation system were the same whether the system was programmed with poly A(−) or poly (A+) RNA. Polypeptides synthesized in the in vitro translation system were electrophoretically similar to those extracted from intact seeds, testimony to the integrity of isolated messenger RNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1985.tb12986.x
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