ISSN:
1436-5073
Keywords:
vitamin B12 (cyanocobalamin)
;
competitive binding assays
;
enzyme-vitamin conjugates
;
vitamin tablet analyses
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new solid-phase enzyme-linked competitive binding assay for vitamin B12 (cyanocobalamin) is described. The assay is based on the competition between analyte B12 molecules and a glucose-6-phosphate dehydrogenase-vitamin B12 conjugate for a limited number of R-protein binding sites immobilized on sepharose particles. After appropriate incubation and washing steps, the enzyme activity bound to the solid-phase is inversely related to the concentration of B12 in the sample. Under optimized conditions, the method can detect B12 in the range of 3×10−10−1×10−8 M (using 100μl sample) with high selectivity over other biological molecules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01197285
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