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  • competitive binding assays  (1)
  • low alkalinity  (1)
  • Springer  (2)
  • American Physical Society (APS)
  • 1985-1989  (2)
  • 1970-1974
Collection
Keywords
Publisher
  • Springer  (2)
  • American Physical Society (APS)
Years
  • 1985-1989  (2)
  • 1970-1974
Year
  • 1
    Electronic Resource
    Electronic Resource
    A solid-phase enzyme-linked assay for vitamin B12 (1989)
    Springer
    Microchimica acta 97 (1989), S. 65-73 
    Details
    ISSN: 1436-5073
    Keywords: vitamin B12 (cyanocobalamin) ; competitive binding assays ; enzyme-vitamin conjugates ; vitamin tablet analyses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new solid-phase enzyme-linked competitive binding assay for vitamin B12 (cyanocobalamin) is described. The assay is based on the competition between analyte B12 molecules and a glucose-6-phosphate dehydrogenase-vitamin B12 conjugate for a limited number of R-protein binding sites immobilized on sepharose particles. After appropriate incubation and washing steps, the enzyme activity bound to the solid-phase is inversely related to the concentration of B12 in the sample. Under optimized conditions, the method can detect B12 in the range of 3×10−10−1×10−8 M (using 100μl sample) with high selectivity over other biological molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01197285
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Phytoplankton communities response to a mine effluent rich in copper (1985)
    Springer
    Hydrobiologia 128 (1985), S. 61-69 
    Details
    ISSN: 1573-5117
    Keywords: phytoplankton ; copper ; mine drainage ; low alkalinity ; reservoirs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of a mine effluent containing high copper levels on the structure of the phytoplankton communities in a chain of low alkalinity and hardness oligotrophic reservoirs situated north of Portugal were studied monthly, during 1981–1982. In the reservoirs under mine influence, a shift in the dominant species have occurred. In contrast with the uncontaminated areas, more than 50% of the algal species developed lower populations. At the most polluted zone, phytoplankton density, biomass and species number were strongly reduced. The mine input stress on the algal communities was well assessed by species number and percentage similarity index.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00008941
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    Paper (German National Licenses)
    Fulltext
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