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  • Analytical Chemistry and Spectroscopy  (846)
  • Cell & Developmental Biology  (705)
  • GENERAL
  • Wiley-Blackwell  (1,551)
  • 1985-1989  (1,179)
  • 1970-1974  (332)
  • 1955-1959  (40)
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  • Wiley-Blackwell  (1,551)
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  • 1
    Electronic Resource
    Electronic Resource
    Differential expression of metastasis-associated cell surface glycoproteins and mRNA in a murine large cell lymphoma (1986)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 31 (1986), S. 305-312 
    Details
    ISSN: 0730-2312
    Keywords: tumor metastasis ; gene expression ; oncogenes ; virus antigens ; glycoproteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A metastatic variant cell subline of the Abelson virus-transformed murine large lymphoma/lymphosarcoma RAW 117 has been selected in vivo ten times for liver colonization. Highly metastatic subline RAW117-H10 forms greater than 200 times as many gross surface liver tumor nodules as the parental line RAW117-P. Analysis of cellular proteins and glycoproteins indicates reduced expression of murine Moloney leukemia virus-associated p15, p30, and gp70, and increased expression of a sialoglycoprotein, gp150, in the highly metastatic H10 cells. Northern analyses of oncogene expression suggested that mRNA of various oncogenes was expressed equally or not expressed in the RAW117 cells of differing metastatic potential. Differential gene expression was examined using a cDNA library of 17,600 clones established from poly A+ mRNA isolated from H10 cells. The cDNA library was screened by the colony hybridization technique using probes made from both RAW117-P and -H10 cells. Approximately 99.5% of these cDNA clones were expressed identically in P and H10 cells. Of the few differentially expressed cDNA clones (approx. 150/17,600), one-half of these were identified as Moloney leukemia virus sequences in a separate probing with a radiolabeled Moloney leukemia virus probe. The remainder of the differentially expressed mRNA detected by colony hybridization of the cDNA library were expressed at higher levels (approx. 1/6) or lower levels (approx. 1/3) in the highly metastatic H10 cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240310408
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of chlorpromazine and its major metabolites by gas chromatography/mass spectrometry: Application to biological fluids (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 707-713 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method for the quantitative determination of chlorpromazine and five of its major metabolites in a single sample of biological fluid in the ng/ml range has been developed utilizing gas chromatography/mass spectrometry with selected ion recording. The assay is highly specific and quantification is accomplished by an inverse stable isotope dilution technique, using deuterium-labeled variants of the compounds as internal standards. In this way the concentrations of chlorpromazine and five of its major metabolites (the sulfoxide, the N-oxide, the monodemethylated, the didemethylated, and the 7-hydroxylated compounds) can be determined in biological fluids. Levels in humans have been measured both in plasma and in red blood cells and are compared to those found in related in vitro studies.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200121207
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of sulfated glycoconjugates on capacitation and the acrosome reaction of bovine and hamster spermatozoa (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 24 (1989), S. 403-413 
    Details
    ISSN: 0148-7280
    Keywords: heparin ; fertilization ; dextran sulfate ; fucose sulfate glycoconjugate ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of sulfated glycoconjugates on the preparation of mammalian sperm for fertilization were investigated. The three sulfated glycoconjugates tested were heparin, dextran sulfate, and the fucose sulfate glycoconjugate (FSG) from the sea urchin egg jelly coat. In vivo, FSG induces the acrosome reaction in sea urchin sperm. Bovine sperm were found to be capacitated by heparin and FSG as judged both by ability of lysophosphatidylcholine (LC) to induce an acrosome reaction and by ability to fertilize bovine oocytes in vitro. The mechanism by which heparin or FSG capacitated bovine sperm appeared similar, since glucose inhibited capacitation by both glycoconjugates. In contrast to effects on bovine sperm, heparin and FSG induced the acrosome reaction in capacitated hamster sperm. When hamster sperm were incubated under noncapacitating conditions, heparin had no effect on capacitation or the acrosome reaction. Three molecular weights (MW) of dextran sulfate (5,000, 8,000, 500,000) were found to capacitate bovine sperm as judged by the ability of LC to induce an acrosome reaction. Whereas bovine sperm incubated with 5,000 or 8,000 M W dextran sulfate fertilized more bovine oocytes than control sperm (P 〈0.05), sperm treated with 500,000 M W dextran sulfate failed to penetrate oocytes. The high-MW dextran sulfate appeared to interact with the zona pellucida and/or sperm to prevent sperm binding. Results suggest that sulfated glycoconjugates may prepare sperm for fertilization across a wide range of species.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120240407
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  • 4
    Electronic Resource
    Electronic Resource
    Primary-particle-beam-induced reduction of silver acetate in glycerol solutions to form a silver mirror (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 24 (1989), S. 733-736 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The liquid SIMS mass spectra of silver acetate dissolved in a glycerol matrix is discussed, with emphasis on the formation of a ‘silver mirror’ on the surface of the glycerol droplet owing to reduction of the silver acetate. Silver clusters containing up to three silver atoms have been observed from this mirrored surface; Ag3+ cluster ions are not observed in the spectrum when conditions are such that the mirror is not formed. For example, use of a slightly oxidizing matrix (o-nitrophenyl octyl ether or m-nitrobenzylalcohol) prevents formation of the ‘mirror’; only Ag+ is sputtered from this surface.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210240905
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  • 5
    Electronic Resource
    Electronic Resource
    N-methylene chemical shifts of N-benzylpyridinium salts (1987)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 25 (1987), S. 560-562 
    Details
    ISSN: 0749-1581
    Keywords: chemical shifts ; N-methylene protons ; N-benzylpyridinium salts ; substituent effects ; addivity relationships ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The chemical shifts of N-benzylpyridinium salts have been measured in D2O, and the substituent induced chemical shifts of the methylene protons are in substantial agreement with those expected from the overall electronic effects of the substituents. An additivity effect of the substituents on the methylene resonance is also observed.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260250622
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  • 6
    Electronic Resource
    Electronic Resource
    Analyse Vibrationnelle et Structurale en Série Aliphatique Saturee. VIIIPour l'article VII dans cette série, voir J. L. Delarbre, L. Maury et L. Bardet, J. Raman Spectrosc. 16, 288 (1985). - Spectres de Vibration de l'acide Glutarique et de ses Sels Alcalins en Solution Aqueuse (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 19 (1988), S. 167-174 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The Raman and infrared spectra of glutaric acid, (CH2)3[COOH(D)]2, and its potassium salts, CH2)3(COO2KHD and CH23COO2K2, in aqueous solution have been investigated in the range 3500-200 cm-1. The analysis of the polarization ratio of the Raman lines indicated that glutaric acid belongs to point group C1 whereas its ions belong to point group Cs. An assignment of the fundamental vibrations is proposed.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250190305
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  • 7
    Electronic Resource
    Electronic Resource
    Analyse Vibrationnelle et Structurale en Série Aliphatique Saturée. IXPour l'article VIII dans cette série, voir Réf. 5. - Spectres de Vibration de l'Acide Pimelique et de ses Sels Alcalins en Solution Aqueuse (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 20 (1989), S. 41-48 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The Raman and infrared spectra of pimelic acid, (CH2)5[COOH (D)]2, and its potassium salts in aqueous solution were recorded from 4000 to 200 cm-1. The two salts belong to the Cs symmetry point group whereas the acid has C1 symmetry. These results are similar to those found previously for lower alkanedloic acids with an odd number of carbon atoms, namely glutaric acid, (CH2)3(COOH)2, and malonic acid, CH2(COOH)2.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250200109
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  • 8
    Electronic Resource
    Electronic Resource
    Application of 15N NMR to a quantitative investigation of tautomerism in some thiolopyridines (1985)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 23 (1985), S. 790-792 
    Details
    ISSN: 0749-1581
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 15N NMR shielding measurements are reported for a series of derivatives of some thiolopyridines. The results obtained are used to provide quantitatively reliable estimates of three tautomeric equilibria involving the pyridine nitrogen atom.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260230923
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  • 9
    Electronic Resource
    Electronic Resource
    Spermiogenesis in the bullfrog (Rana catesbeiana): A study of cytoplasmic events including cell volume changes and cytoplasmic elimination (1988)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 198 (1988), S. 303-319 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The process by which spermatid cytoplasmic volume is reduced and cytoplasm eliminated during spermiogenesis was investigated in the bullfrog Rana catesbeiana. At early phases of spermiogenesis, newly formed, rounded spermatids were found within spermatocysts. As acrosomal development, nuclear elongation, and chromatin condensation occurred, spermatid nuclei became eccentric within the cell. A cytoplasmic lobe formed from the caudal spermatid head and flagellum and extended toward the seminiferous tubule lumen. The cytoplasmic lobe underwent progressive condensation whereby most of its cytoplasm became extremely electron dense and contrasted sharply with numerous electron-translucent vesicles contained therein. At the completion of spermiogenesis, many spermatids with their highly condensed cytoplasm still attached were released from their Sertoli cell into the lumen of the seminiferous tubule. There was no evidence of the phagocytosis of residual bodies by Sertoli cells. Because spermatozoa are normally retained in the testis in winter and are not released until the following breeding season, sperm were induced to traverse the duct system with a single injection of hCG. Some spermatids remained attached to their cytoplasm during the sojourn through the testicular and kidney ducts; however, by the time the sperm reached the Wolffian duct, separation had occurred. The discarded cytoplasmic lobe (residual body) appeared to be degraded within the epithelium of the Wolffian duct. It was determined that the volume of the spermatid was reduced by 87% during spermiogenesis through a nuclear volume decrease of 76% and cytoplasmic volume decrease of 95.3%.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051980305
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  • 10
    Electronic Resource
    Electronic Resource
    Histogenesis of Swiss white mouse secondary palate from nine and one-half days to fifteen and one-half days In utero. I. Epithelial-mesenchymal relationships - light and electron microscopyThis work was supported by Research grant DE 03002 from the National Institute of Dental Research, NIH. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 435-449 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Development of the secondary palate in Swiss white mouse embroyos was studied from age nine-and-one-half days in utero to the stage of mesenchymal coalescence in the secondary palate (approximately fifteen-and-one-half days). The greatest changes observed occur in the mesenchyme. At early stages, mesenchymal cells underlying oral ectoderm of the head are few and only occasionally contact the ectoderm. Electron micrographs show large intercellular spaces between the ectodermal cells. As embryogenesis continues, the mesenchymal cells become more numerous, closer to each other and closer to the epithelium. Just prior to horizontal transposition of shelves, the mesenchymal cells spread farther from each other and from the palatal epithelium and epithelium of the palatal tip becomes stretched. Ultrastructurally the intercellular spaces between epithelial cells of the palate tip have become much smaller. Some mitochondria in some epithelial cells are swollen and have clear matrices and distorted cristae. The shelves become horizontal and meet in the midpalate. Cells with degeneration bodies are seen in the epithelial seam. The seam undergoes autolysis and is replaced by mesenchyme. The morphological changes described, particularly in the mesenchyme, may play an important role in determining the effect of various teratogens at different stages of palatal development. The changes in both mesenchyme and epithelial cells in the later stages may constitute part of the process of preparing shelves for fusion as postulated by Pourtois ('66).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051300404
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