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  • Chemistry  (4,421)
  • Cell & Developmental Biology  (733)
  • Chemical Engineering  (725)
  • 1985-1989  (3,361)
  • 1975-1979  (1,793)
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Keywords
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Electron microscopic investigation of polyamidohydrazide solutions (1985)
    Weinheim : Wiley-Blackwell
    Acta Polymerica 36 (1985), S. 624-627 
    Details
    ISSN: 0323-7648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Unter Hernziehung unterschiedlicher Präparationsmethoden wurde die Struktur verdünnter und konzentrierter (0,01 bis 9,8%) Lösungen des starkettigen Polyamidohydrazid (PAH) mittels Elektronenmikroskopie in zwei Lösungsmitteln (DMSO und DMAA) untersucht. Beginnend mit der Konzentration 0,01% erfolgt unterschiedliche Strukturierung in diesen Lösungsmitteln, die bezüglich des Polymers eine unterschiedliche Güte aufweisen. Die Ergebnisse wurden früheren Untersuchungen zur Rheologie konzentrierter PAH-Lösungen in den genannten Lösungsmitteln gegenüber gestellt.
    Notes: The structure of dilute and concentrated solutions (0.01-9.8%) of rigid-chain polyamidohydrazide (PAH) in dimethylsulphoxide and dimethylacetamide was investigated by electron microscopy using various preparation techniques. Starting with a 0.01% concentration, PAH shows two distinct types of structuration in the solvents. The results are compared with previously studied rheological properties of PAH in these solvents.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/actp.1985.010361110
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of chlorpromazine and its major metabolites by gas chromatography/mass spectrometry: Application to biological fluids (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 707-713 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method for the quantitative determination of chlorpromazine and five of its major metabolites in a single sample of biological fluid in the ng/ml range has been developed utilizing gas chromatography/mass spectrometry with selected ion recording. The assay is highly specific and quantification is accomplished by an inverse stable isotope dilution technique, using deuterium-labeled variants of the compounds as internal standards. In this way the concentrations of chlorpromazine and five of its major metabolites (the sulfoxide, the N-oxide, the monodemethylated, the didemethylated, and the 7-hydroxylated compounds) can be determined in biological fluids. Levels in humans have been measured both in plasma and in red blood cells and are compared to those found in related in vitro studies.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200121207
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  • 3
    Electronic Resource
    Electronic Resource
    Electronic computer solution for the MPN equation used in the determination of bacterial populations (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 1335-1335 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260180912
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  • 4
    Electronic Resource
    Electronic Resource
    Large-scale production of monoclonal antibodies in suspension culture (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 32 (1988), S. 993-1000 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Monoclonal antibodies are being manufactured for clinical trials in suspension culture at the 1300-L scale. Suspension culture offers some advantages relative to high-density mammalian cell culture methods; in particular, the ability to closely monitor the behavior of cells in a homogeneous environment. Computer control and on-line mass spectrography of exit gases provide instantaneous information about the culture metabolic activity. Air sparging and agitation by marine impeller provide aeration sufficient to maintain a constant dissolved oxygen tension at cell concentrations up to 5.0 × 106 cells/mL without causing apparent cell damage.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260320807
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  • 5
    Electronic Resource
    Electronic Resource
    A microfibril-generating factor from the cellulase of Trichoderma reesei (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 31 (1988), S. 321-327 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A combination of ionic strength reduction and diafiltration of Trichoderma reesei cellulate complex through a hollow fiber apparatus of 5000 molecular weight (MW) cutoff and subsequent passage of filtrate over a Spherogel-TSK 3000-SW column provided extracts that had the ability to generate microfibrils in filter paper and to disrupt filter paper and corn leaf tissue. Milligram quantities of material obtained from these extracts released small amounts of soluble carbohydrate from filter paper, required ferric iron for increased activity, and contained amino acids. Short fiber formation and disruption of filter paper during interaction with these extracts was enhanced by prior acid treatment and eliminated by prior base treatment. The amount of soluble carbohydrate hydrolyzed in 24 h from filter paper by whole cellulase complex was not changed by first disrupting the substrate with the extracts.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260310407
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  • 6
    Electronic Resource
    Electronic Resource
    Structure and properties of copolymers of vinylcyclohexane and α-methylvinylcyclohexane with acrylonitrile (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 23 (1979), S. 2989-2997 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A study has been made of the structure and properties of alternating equimolar and random copolymers of vinylcyclohexanne and α-methylvinylcyclohexane with acrylonitrile using infrared and 1H-NMR spectroscopy methods, as well as x-ray diffraction and differential thermal analyses. The spectral methods allowed an estimation of the composition and distribution of comonomer units in the copolymer macrochains. The thermal stability of alternating copolymers was found to be higher than that of polyacrylonitrile. Alternating and random copolymers are amorphous products with various dimensions of crystallites. Information on the molecular motion intensity and temperatures of structural transitions in copolymer macrochains was obtained by the spin-probe technique.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1979.070231017
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of local controlled release of sodium fluoride on the stimulation of bone growth (1989)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 23 (1989), S. 571-589 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: This study was performed to test the feasibility of using a biodegradable delivery system for the local controlled release of sodium fluoride as a mechanism for the stimulation of local bone growth. Sodium fluoride grains were mixed with poly-DL-lactic acid (PLA), and the mixture was then dissolved in acetone. After driving off the solvent, sheets of this material were rolled into rods 3.2 mm in diameter and 28.6 mm long. These were inserted into the intramedullary canals of the right femora of adult New Zealand White rabbits. A sham implant, made the same way but containing no fluoride, was inserted into the contralateral left leg. The effects of three different concentrations of sodium fluoride, 0.125 g NaF/g PLA, 0.250 g NaF/g PLA, and 0.500 g NaF/g PLA, were determined after a 4-week implantation period. In a second set of experiments, the concentration was fixed at 0.500 g NaF/g PLA with implantation durations of 4, 8, and 16 weeks. Expressed in terms of property ratios (experimental/control), bending strengths of the femora were significantly raised in most groups with the notable exception of Group 3 (0.500 g NaF/g PLA, 4 weeks). Cross-sectional area ratios in the distal femora were increased in all groups compared to right/left values for a normal unoperated group (p 〈 0.05), with the highest ratio being 1.50 (SD = 0.44) for Group 4 (0.500 g NaF/g PLA, 8 weeks). Only Group 4 animals showed significant (p 〈 0.05) increases in midshaft area ratio and none had responses proximally. Growth rates, measured by sequential fluorochrome labeling of bone followed the area ratio data and were only significant for the distal femora.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820230604
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  • 8
    Electronic Resource
    Electronic Resource
    Preparation of Mikanecic EsterThe systematic name of mikanecic acid is 4-vinyl-cyclohex-1-ene-1,4-dicarboxylic acid; it is used in the experimental Part. and its precursor, 1,3-butadiene-2-carboxylic ester (1975)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 58 (1975), S. 2061-2073 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Eine thermische (a) und eine reduktive (b) Methode zur Herstellung von Mikanez-Estern wird beschrieben. Bei der ersten gelang der Nachweis des monomeren Vorläufers, 1,3-Butadien-2-carboxylester. (a)trans/cis-Gemische von Methyl resp. Äthyl 2-Brom-1-methylcyclopropancarboxylat (14/15 resp. 16/17), mit Tri-n-butylzinnhydrid aus den entsprechenden 2,2-Dibrom-1-methylcyclopropancarboxylaten 12 resp. 13 hergestellt, wurden bei 480°/1,7 Torr pyrolysiert. Die bei -78° daraus abgefangenen Kondensate bestanden aus den 1,3-Butadien-2-carboxylaten 5 und 6, im Falle des Äthylesters 6 durch seine 1H-NMR.-Signale charakterisiert und als Addukt (19) mit 4-Phenyl-1,2,4-triazolin-3,5-dion (18) abgefangen (55%). Beim Erwärmen der Kondensate dimerisierten die Dienester 5 resp. 6, so dass sich Dimethyl resp. Diäthyl Mikanezat (9 resp. 10) isolieren liess (67 resp. 100%).(b)Behandlung von Methyl 2(E)-2-Methyl-2-butenoat (20) mit 2 Äquivalenten N-Bromsuccinimid gab (25%) Methyl 2(Z)-4-Brom-2-brommethyl-2-butenoat (21). (Mit 3 Äquivalenten entstand ein Stereomerengemisch 2(Z)- und 2(E)-4,4-Dibrom-2-brommethyl-2-butenoat (22 und 23)). Reduktion des Dibromesters 21 mit Zink in Tetrahydrofuran, Methanol oder Eisessig ergab (50%) Dimethyl Mikanezat (9). Von einer Iodolaktonisierung der aus 9 durch Verseifung hergestellten Mikanezsäure (8) erhielt man 4-Iodo-7-oxo-1-vinyl-6-oxabicyclo[3.2.1]octan-4endo-carbonsäure (24), dessen Bildungsleichtigkeit und IR.-Bande bei 1780 cm eine unabhängige Bestätigung dafür liefert, dass Mikanezsäure (8) durch Kopf-zu-Kopf-Diels-Alder-Dimerisierung von 1,3-Butadien-2-carbonsäure (4) entsteht.Aus Methyl (E)-2-Methyl-2-pentenoat (25) wurde auf gleiche Weise, d.h. über Dibromierung zu 27 und Reduktion mit Zink in Äther, Dimethyl 3,2′-Dimethylmikanezat (29) gewonnen. Die Multiplizität des 1H-NMR.-Signals von H—C(3) in 29 bestätigt, wiederum unabhängig, dass die Dimerisierung des Dienesters (in diesem Fall 28) in Kopf-zu-Kopf-Diels-Alder-Weise stattfindet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19750580721
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of trichroic infrared absorption in solids. III. Phase I poly(vinylidene fluride) (1986)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 24 (1986), S. 2541-2551 
    Details
    ISSN: 0887-6266
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Trichroic infrared absorption has been measured for oriented phase I poly(vinylidene fluoride). In addition to intensity differences, frequency shifts in the plane transverse to the draw direction are apparent. Shifts up to 10 cm-1 in one-way drawn phase I are found for the CF2 stretching vibration (880 cm-1) between the direction normal to the film surface (thickness direction) and the remaining directions. Annealing the highly oriented films produces no major changes in the unique three-dimensional structure. A model based on crystal defect structures is proposed to explain the unusual frequency shift behavior in the phase I PVDF. Possible conformations and a mechanism of formation of the defects are discussed.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/polb.1986.090241111
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  • 10
    Electronic Resource
    Electronic Resource
    Differential expression of metastasis-associated cell surface glycoproteins and mRNA in a murine large cell lymphoma (1986)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 31 (1986), S. 305-312 
    Details
    ISSN: 0730-2312
    Keywords: tumor metastasis ; gene expression ; oncogenes ; virus antigens ; glycoproteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A metastatic variant cell subline of the Abelson virus-transformed murine large lymphoma/lymphosarcoma RAW 117 has been selected in vivo ten times for liver colonization. Highly metastatic subline RAW117-H10 forms greater than 200 times as many gross surface liver tumor nodules as the parental line RAW117-P. Analysis of cellular proteins and glycoproteins indicates reduced expression of murine Moloney leukemia virus-associated p15, p30, and gp70, and increased expression of a sialoglycoprotein, gp150, in the highly metastatic H10 cells. Northern analyses of oncogene expression suggested that mRNA of various oncogenes was expressed equally or not expressed in the RAW117 cells of differing metastatic potential. Differential gene expression was examined using a cDNA library of 17,600 clones established from poly A+ mRNA isolated from H10 cells. The cDNA library was screened by the colony hybridization technique using probes made from both RAW117-P and -H10 cells. Approximately 99.5% of these cDNA clones were expressed identically in P and H10 cells. Of the few differentially expressed cDNA clones (approx. 150/17,600), one-half of these were identified as Moloney leukemia virus sequences in a separate probing with a radiolabeled Moloney leukemia virus probe. The remainder of the differentially expressed mRNA detected by colony hybridization of the cDNA library were expressed at higher levels (approx. 1/6) or lower levels (approx. 1/3) in the highly metastatic H10 cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240310408
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