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  • Phycomyces  (3)
  • 65H10  (2)
  • 1985-1989  (5)
  • 1975-1979
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Computing 38 (1987), S. 133-141 
    ISSN: 1436-5057
    Keywords: 65H10 ; Nonlinear systems ; Quasi-Newton methods ; sparse matrices ; factorization of matrices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Wir stellen in dieser Arbeit ein Verfahren vom Quasi-Newton-Typ für große, dünnbesetzte nichtlineare Gleichungssysteme vor, das die QR-Faktorisierung der näherungsweisen Jacobi-Matrix benutzt. Das Verfahren gehört zu einer allgemeinen Klasse von Algorithmen, für die wir die lokale Konvergenz beweisen. Einige numerische Experimente deuten auf die Verläßlichkeit des neuen Algorithmus hin.
    Notes: Abstract In this paper we present a Quasi-Newton type method, which applies to large and sparse nonlinear systems of equations, and uses the Q-R factorization of the approximate Jacobians. This method belongs to a more general class of algorithms for which we prove a local convergence theorem. Some numerical experiments seem to confirm that the new algorithm is reliable.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Computing 37 (1986), S. 59-70 
    ISSN: 1436-5057
    Keywords: 65H10 ; Nonlinear equations ; projection methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Wir betrachten eine Block-Version des Nichtlinearen Projektions-Verfahrens mit einer optimalen Kontrolle. Dieses Verfahren wird zur Lösung von Gleichungssystemen verwendet, bei denen die Gleichungsanzahl kleiner oder gleich der Anzahl der Unbekannten ist. Ein lokaler Konvergenzsatz wird bewiesen. Ein numerischer Vergleich mit dem zyklischen Nichtlinearen Projektions-Verfahren wird angestellt.
    Notes: Abstract We consider a block version of the Nonlinear Projection Method under an optimal control. This method is applied to the solution of systems of equations where the number of equation is less than or equal to the number of unknowns. A local convergence theorem is proved. We present a numerical comparison with the cyclic Nonlinear Projection Method.
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  • 3
    ISSN: 1432-072X
    Keywords: Mucor ; Phycomyces ; Diaminobutanone ; Polyamines ; Dimorphism ; Phorogenesis ; Ornithine decarboxylase ; Fungal differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about a decrease in ODC and polyamine levels. In these conditions, the aerobic shift produced only a weak increase in ODC activity and no change in polyamine levels. DAB also blocked phorogenesis in M. rouxii and in Phycomyces blakesleeanus. At the effective concentrations DAB did not affect cell growth of either fungus. It is suggested that low, constant levels of ODC and polyamines are necessary for cell growth, and that high transient levels are required during the differentiative steps. DAB, at the concentrations used, affects this last process, but does not interfere with the maintenance level of polyamines.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 212 (1988), S. 375-377 
    ISSN: 1617-4623
    Keywords: Phycomyces ; Transposon Tn5 ; Kanamycin ; Fungal transformation ; Fungal promoters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A plasmid, carrying the Tn5 gene for kanamycin resistance lacking its own promoter, has successfully been used in the selection of DNA sequences of the fungus Phycomyces blakesleeanus having promoter activity in Escherichia coli. Many of these sequences were also effective in promoting resistance to kanamycin when the corresponding chimeric plasmids were introduced in the fungus via spheroplast transformation. The selected phenotype was easily propagated through vegetative spores and behaved as a stable character since it was not appreciably lost in the absence of selection.
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  • 5
    ISSN: 1615-6102
    Keywords: Vesicles ; Phycomyces ; Calcium ionophore ; Chitin synthetase ; Invertase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hyphal elongation, chitin synthesis in vivo, and invertase secretion inPhycomyces blakesleeanus were all inhibited almost instantly by the addition of 5–10 μM calcium ionophore A 23187. Protein biosynthesis was inhibited in these conditions by 30–50%. The ionophore did not affect cell respiration for at least 40 min. Effect on chitin biosynthesis was not due to alterations of the chitin synthetase levels or its activity; nor to impairement in GlcNAc metabolism. In drug-treated cells the number of apical vesicles was severely reduced even at very short periods of incubation, and these low numbers remained constant for at least 60 min of incubation with the ionophore. We suggest that the ionophore collapses the cellular calcium gradient and/or interferes with the normal electrical transhyphal current. As a consequence, formation and migration of apical vesicles are inhibited. These results are further evidence of the role of vesicles in fungal tip growth and exhibit the fact that active chitin synthetase is short-lived in vivo demanding its continuous supply by chitosomes to the cell surface.
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