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  • Alnus rubra  (1)
  • Fracture callus cartilage  (1)
  • Springer  (2)
  • Periodicals Archive Online (PAO)
  • 1985-1989  (1)
  • 1975-1979  (1)
Collection
Keywords
Publisher
  • Springer  (2)
  • Periodicals Archive Online (PAO)
Years
  • 1985-1989  (1)
  • 1975-1979  (1)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Purification of alkaline phosphatase from extracellular vesicles of fracture callus cartilage (1978)
    Springer
    Calcified tissue international 25 (1978), S. 85-92 
    Details
    ISSN: 1432-0827
    Keywords: Fracture callus cartilage ; Matrix vesicles ; Alkaline phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Extracellular matrix vesicles from fracture callus cartilage were isolated by differential centrifugation and resolved by equilibrium centrifugation on a discontinuous sucrose gradient into two bands. The phosphohydrolytic activity towards p-nitrophenyl phosphate, tetrasodium pyrophosphate and adenosine triphosphate was distributed similarly after differential and equilibrium centrifugation suggesting the association of this activity with the matrix vesicles. The two bands isolated by equilibrium centrifugation of the partially purified vesicular preparation demonstrated high levels of alkaline phosphatase activity. Observed with an electron microscope, the 1.07–1.14 g/cm3 band from the gradient was enriched in electron luscent matrix vesicles while the 1.27 g/cm3 band contained electron dense matrix vesicles. Enzymatic analysis of the 1.27 g/cm3 band indicated a slight contamination due to the presence of mitochondria and lysosomes while the 1.07–1.14 g/cm3 band gave no enzymatic indication of subcellular contamination. A phosphohydrolytic enzyme active towards p-nitrophenyl phosphate, tetrasodium pyrophosphate and adenosine triphosphate was purified from the 1.07–1.14 g/cm3 fraction by DEAE-cellulose column chromatography. Electron micrographs of callus cartilage sections demonstrated densification of the plasma membrane and matrix vesicles following substrate incubation withβ-glycerophosphate or tetrasodium pyrophosphate. The histochemical and biochemical data indicate that a phosphatase, with multiple substrate specificity, is a component of fracture callus cartilage matrix vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02010755
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of indole compounds secreted byFrankia HFPArI3 in defined culture medium (1989)
    Springer
    Plant and soil 118 (1989), S. 205-209 
    Details
    ISSN: 1573-5036
    Keywords: actinorhizal ; Alnus rubra ; auxin ; Frankia ; IAA ; indole-3-acetic acid ; indole-3-ethanol ; nodule secretion ; phytohormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole compounds secreted byFrankia sp. HFPArI3 in defined culture medium were identified with gas chromatography-mass spectrometry (GC-MS). WhenFrankia was grown in the presence of13C(ring-labelled)-L-tryptophan,13C-labelled indole-3-acetic acid (IAA), indole-3-ethanol (IEtOH), indole-3-lactic acid (ILA), and indole-3-methanol (IMeOH) were identified. High performance liquid chromatography (HPLC) and GC-MS with selected ion monitoring were used to quantify levels of IAA and IEtOH inFrankia culture medium. IEtOH was present in greater abundance than IAA in every experiment. When no exogenous trp was supplied, no or only low levels of indole compounds were detected. Seedling roots ofAlnus rubra incubated in axenic conditions in the presence of indole-3-ethanol formed more lateral roots than untreated plants, indicating that IEtOH is utilized by the host plant, with physiological effects that modify patterns of root primordium initiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02232808
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    Paper (German National Licenses)
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