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  • Gas chromatography  (4)
  • Glycoproteins  (2)
  • Springer  (6)
  • American Institute of Physics
  • International Union of Crystallography (IUCr)
  • Molecular Diversity Preservation International
  • Periodicals Archive Online (PAO)
  • Springer Nature
  • Wiley-Blackwell
  • 1985-1989  (6)
  • 1975-1979
Collection
Keywords
Publisher
  • Springer  (6)
  • American Institute of Physics
  • International Union of Crystallography (IUCr)
  • Molecular Diversity Preservation International
  • Periodicals Archive Online (PAO)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Characterization of a proteinaceous extracellular coat synthesized by the “slime” variant of Neurospora crassa (1989)
    Springer
    Archives of microbiology 152 (1989), S. 25-32 
    Details
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; “Slime” variant ; Cell coat ; Exocellular proteins ; Glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of the “slime” strain of Neurospora crassa synthesize a coherent extracellular material which remains attached to the cell surface, but is released into the liquid medium by shaking. The material was purified and studied by different criteria. By electron microscopy it appears as long wavy sheets which strongly bind concanavalin A, but not wheat germ agglutinin, and maintain their integrity in the absence of structural polysaccharides. Analysis of the purified material revealed that it was free of contaminating membranes; it contained more than 70% protein, 1% neutral sugars (glucose, mannose, fucose and galactose), less than 2% lipids and ca. 4% not-characterized hexosaminelike compounds. Its polypeptide pattern as determined by PAGE was complex. The significance of this material is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447007
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  • 2
    Electronic Resource
    Electronic Resource
    Self-assembly properties of the proteinaceous coat secreted by the “slime” variant of Neurospora crassa (1989)
    Springer
    Archives of microbiology 152 (1989), S. 33-38 
    Details
    ISSN: 1432-072X
    Keywords: Neurospora crassa ‘Slime” variant ; Cell coat ; Glycoproteins ; Self-assembling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The proteinaceous extracellular material (PEM) synthesized by the cells of the ‘slime” strain of Neurospora crassa (see Martinez et al. 1989) was solubilized by treatment with urea or guanidine. Removal of these chemicals by dialysis, caused reassembly of the solubilized proteins into material with the same microscopic appearance as the original PEM. Polypeptide patterns from both native and reassembled structures were identical. Dialysis-mediated reassembly of the solubilized proteins appeared to be dependent on both concentration of the soluble macromolecules and time. Gel chromatography of PEM solubilized with different agents revealed two discrete populations of complexes with molecular masses of 1,500 and 500 kDa respectively. These were able to reassemble into lamellar structures with a variable degree of efficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447008
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  • 3
    Electronic Resource
    Electronic Resource
    Simultaneous distillation-extraction (SDE) method in the qualitative and quantitative GC analysis of cheese volatile components (1988)
    Springer
    Chromatographia 25 (1988), S. 861-864 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Simultaneous distillation-extraction ; Cheese volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simultaneous distillation-extraction apparatus proposed by Godefroot has been used for the GC study of volatile components of cheese. 5–10 g of cheese provide a fraction that can be directly injected into a GC or a GC/MS. Two capillary columns (SE-30 and SP-1000) were evaluated, the second one being adequate for quantitative determinations. Camphor was used as internal standard. The coefficients of variation ranged from 1.4 to 11.8. The technique can be used as a fast method for the characterization of cheeses from their volatile component concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02311418
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  • 4
    Electronic Resource
    Electronic Resource
    Gas chromatographic analysis of free fatty acids and glycerides of milk fat using tetramethylammonium hydroxide as catalyste (1986)
    Springer
    Chromatographia 21 (1986), S. 37-40 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Preparation of methyl esters ; Tetramethylammonium hydroxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Tetramethylammonium hydroxide in methanol has been proposed as a catalyst for preparing methyl esters of both free fatty acids and glycerides of fats. Nevertheless, procedures normally recognized as suitable can be inadequate when the fat under study contains noticeable amounts of low relative molecular mass esters. The method using tetramethylammonium hydroxide has been examined and modified in order to analyze milk fats by capillary GC. The pyrolysis methylation of free fatty acids in capillary injectors has been also studied. The modified method was satisfactory for both neutral and acid milk fats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310592
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of the components of aldoses in a tautomeric equilibrium mixture as their trimethylsilyl ethers by capillary gas chromatography (1987)
    Springer
    Chromatographia 23 (1987), S. 43-46 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Carbohydrate TMS-ethers ; Tautomeric equilibrium mixtures of aldoses ; MS and NMR identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Equilibrium tautomers of aldopentoses (arabinose, ribose, xylose and lyxose) and aldohexones (allose, altrose, gulose, idose and talose) have been analyzed as their trimethyl-silyl ethers by capillary GC. Pentoses were separated on SE-54 and hexoses on OV-225. The four components of the tautomer equilibrium mixture have been identified using GC/MS and NMR data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02310417
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  • 6
    Electronic Resource
    Electronic Resource
    Chromatographic determination of lactulose (1987)
    Springer
    Chromatographia 23 (1987), S. 132-136 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Column liquid chromatography ; Thin-layer chromatography ; Analysis of lactulose in carbohydrate mixtures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Lactulose is a disaccharide with important implications in medicine, nutrition and dairy technology. It has to be determined usually as a minor component in complex matrices containing many other sugars. This paper describes the different chromatographic techniques (planar, liquid and gas chromatography) which have been proposed for the separation and quantification of lactulose in mixtures with other carbohydrates; the advantages of each technique are compared and some features where more research is necessary are outlined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02312889
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