ISSN:
1744-313X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Methodologies are described for the production and characterization of monoclonal antibodies to human haemoglobin. Three monoclonal antibodies are described, two of which recognize distinct determinants on the α-chain subunit. A third monoclonal antibody, Hb-2d, recognizes a determinant expressed on human β-chain. The Hb-2d determinant is shared by human and baboon haemoglobins, but is not expressed by haemoglobins from beef, goose, pig, rabbit, sheep, dog, rat or mouse. Monoclonal antibody Hb-2d will bind to haemoglobin A2 but not to foetal haemoglobin suggesting that δ- but not γ-chain also expresses the Hb-2d determinant. The results of testing a limited panel of human haemoglobin variants is presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1744-313X.1985.tb00847.x
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