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  • Articles  (6)
  • Cell & Developmental Biology  (6)
  • CHEMISTRY
  • Physics
  • 1985-1989  (6)
  • 1975-1979
  • 1970-1974
  • Natural Sciences in General  (6)
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  • Articles  (6)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 229-230 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: In problems of air or water pollution, or in evaluation of exposure to pathogenic dusts from biological specimens ( e.g., asbestos fibers) some typical particles can be used as tracers. As far as they have sufficiently typical properties (shape, color, anisotropy,…) particles are rapidly recognized and quantified by light microscopy (LM), even if they are very scarce or dispersed among large amounts of unsignificant ones. For accurate characterization, analytical electron microscopy (scanning or transmission) is required, but cannot be efficiently applied for low concentrations of particles. A technique using a high precision object-marker under LM has been developed in the past by Jedwab (1975) to be used with SEM, but there is actually no equivalent for TEM. Such a technique is proposed in this paper. Its major interest resides in the greater amount of analytical data available for one single particle (high magnification morphology, crystallographic structure, chemistry). Practical results were obtained with asbestos fibers and bodies recovered from biological specimens, but the technique can be extended to many other problems concerning micron-sized particles.
    Additional Material: 2 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 263-272 
    ISSN: 0741-0581
    Keywords: High Tc superconductors ; Electron microscopy ; HREM image stimulations ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Thin films of the superconductive oxide YBa2Cu3O7-x have been made by electron-beam coevaporation of the metals in an oxygen atmosphere onto single-crystal {001}-oriented SrTiO3 and yttria-stabilized zirconia (YSZ) substrates. The oxide films were superconducting in the as-deposited state (Tc = 81-83K, Jc = 106 A/cm2 at 4.2K). Bright-field imaging, selectedarea diffraction (SAD), and high-resolution imaging in the transmission electron microscope were used to characterize the microstructure of these films. All of the films were polycrystalline. On SrTiO3 the films were oriented, for the most part, with {110} parallel to the substrate surface. On YSZ, two microstructures were observed: one with smaller rectangular grains oriented with (100) or (010) parallel to the substrate surface and the other with (001) parallel to the surface (i.e., c-axis up).
    Additional Material: 10 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 2 (1985), S. 519-520 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Additional Material: 2 Ill.
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  • 4
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Additional Material: 2 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 2 (1985), S. 533-546 
    ISSN: 0741-0581
    Keywords: Reflection electron microscopy (REM) ; Cross-sections ; Interfaces ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Reflection electron microscopy (REM) is shown to be applicable to the testing of the quality of AlxGa1-xAs/GaAs layer structures. Cross-sectional images of quantum well structures with layer thickness down to 1 nm can be obtained. The practical aspects of the REM technique are presented. The most important advantages and drawbacks of cross-sectional REM are discussed in detail.
    Additional Material: 10 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 391-399 
    ISSN: 0741-0581
    Keywords: Ultracryomicrotomy ; Immunogold ; Cultured cells ; Negative staining ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: In spite of the fact that the preembedding method is satisfactory for the ultrastructural localization of cytoskeletal proteins, there is a need for a localization method that retains the cells' ground substance, delicate filament arrangements, and membrane-filament interactions and provides a good delineation of ultrastructural detail. Ultracryomicrotomy, a resinless sectioning method, can combine good morphology with optimal antibody labeling. Until now, however, it has not been possible to section cell monolayers parallel to their plane of growth. This is a prerequisite for the localization of proteins along segments of filaments, contained within the section thickness. We describe such a method and give a first appreciation of its potential for antibody localization studies of cytoskeletal proteins. The method consists of seeding cells on a parallel 0.75-mm-thick gelatin substrate that can later be cut and used as a mounting block. An adapted negative staining has yielded a very useful delineation of the well-preserved structures within the cells, even in combination with immunogold labeling. The latter has been in its indirect version less satisfactory in dense microfilament bundles because of penetration problems, and more satisfactory on microtubules. Clearly, the penetration properties of gold probes will have to be improved before this method will become widely applicable. The availability of a sectioning method like this will provide the basis for further progress. There will be many cases which will justify the use of this relatively more difficult approach.
    Additional Material: 4 Ill.
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