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  • 1985-1989  (4)
  • 1980-1984  (6)
  • 1955-1959  (5)
  • 1
    Electronic Resource
    Electronic Resource
    THE ENZYMATIC SYNTHESIS OF SHIKIMIC ACID FROM D-ERYTHROSE-4-PHOSPHATE AND PHOSPHOENOLPYRUVATE1,2,3 (1955)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 77 (1955), S. 4943-4944 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja01623a089
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  • 2
    Electronic Resource
    Electronic Resource
    THE ENZYMATIC SYNTHESIS OF ANTHRANILIC ACID FROM SHIKIMIC ACID-5-PHOSPHATE AND L-GLUTAMINE1 (1959)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 81 (1959), S. 1772-1773 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja01516a070
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  • 3
    Electronic Resource
    Electronic Resource
    Simulation of Biodegradation and Sorption Processes in Ground Water (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 26 (1988), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A one-dimensional, finite-difference model for simulating biodegradation and sorption processes in saturated porous media is developed. The model formulation is general and capable of accommodating a variety of boundary conditions and process theories. Advective and dispersive transport of a substrate and an electron acceptor are considered. Aerobic biodegradation is modeled using a modified Monod function; anaerobic biodegradation is modeled using Michaelis-Menten kinetics. In addition, first-order degradation is allowed for both substances. Sorption can be incorporated using linear, Freundlich, or Langmuir equilibrium isotherms for either substance. Dirichlet, Neumann, or Cauchy boundary conditions are allowed, where they are constant in time, or include first-order degradation. The resulting nonlinear problem is solved using a Newton-Raphson iterative technique. Model verification is achieved through a series of simulations, two of which arc included. In addition, three applications demonstrate the utility of the model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1988.tb00414.x
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  • 4
    Electronic Resource
    Electronic Resource
    Gene for M1 subunit of ribonucleotide reductase is amplified in hydroxyurea-resistant hamster cells (1987)
    Springer
    Somatic cell and molecular genetics 13 (1987), S. 221-233 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The hydroxyurea-resistant Chinese hamster cell line 600H has been shown to have greatly elevated quantities of ribonucleotide reductase. This increase in enzyme activity is due to an increased level of both the M1 and M2 subunit activities. The M1 subunit has been purified from the 600H cell line and shown to consist of a series of six protein spots with apparent molecular weights of 88,000 daltons, but with varying isoelectric points in the range of pH 6.5–7.0. Western blot analyses with antisera against the M1 and M2 proteins indicated that both subunit proteins are present in elevated quantities in the 600H cell line when compared to the wild-type V79 cell line. Southern blot analyses with genomic DNA from the series of stepwise-selected hydroxyurea-resistant cell lines leading to 600H showed that, in latter steps of selection, genomic sequences homologous to a mouse M1 cDNA have undergone a fivefold amplication. This was accompanied by a four-to eightfold increase in the single M1 homologous mRNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01535204
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on temperature-sensitive mutants of Chinese hamster ovary cells affected in DNA synthesis (1980)
    Springer
    Somatic cell and molecular genetics 6 (1980), S. 567-582 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract DNA synthesis in two mutants of Chinese hamster overy cells,ts 13A andts 15C, which were temperature sensitive for growth, was found to be shut off rapidly at the nonpermissive temperature. The mutants did not complement each other and thets lesion was not located on the X chromosome. Both isolates were found to be considerably more sensitive to the alkylating agents, ethylmethanesulfonate (EMS) and methylmethanesulfonate (MMS), as compared to the parental cells, but showed normal sensitivity to UV irradiation. The mutants also showed interesting differences in their response to EMS-induced mutation frequencies at the ouabain-resistant and thioguanine-resistant loci. At high survival (50%) the frequencies of mutations at these genetic loci were markedly low in thets mutants as compared to the parental cells. Ints * revertants isolated from the mutants, thets phenotype and the increased sensitivity to EMS and MMS were affected simultaneously, indicating that both these characteristics resulted from a single genetic lesion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538638
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  • 6
    Electronic Resource
    Electronic Resource
    Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA (1980)
    Springer
    Somatic cell and molecular genetics 6 (1980), S. 333-348 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The conditions necessary to achieve high frequency transfer of the thymidine kinase and dihydrofolate reductase genes from hamster cells into mouse cells were investigated. Of the parameters examined, the length of adsorption time, input gene dosage, and treatment with dimethylsulfoxide (DMSO) were found to significantly alter the transfer frequency using either metaphase chromosomes or purified DNA as the transfer vehicle. With the mouse cell line as a recipient, the optimal adsorption period for DNA or chromosomes from MtxRIII cells was found to vary from 8 to 16 h in those experiments where the recipient cells were subsequently treated with DMSO. Without DMSO, similar frequencies could be obtained by extending the period of adsorption. Increasing the dosage of DNA or chromosomes resulted in an almost linear increase in the number of transformants. The optimal conditions for transfer did not significantly differ for the two genes studied. On the average, the optimal conditions yielded 1.5×103 transformants per 107 recipient cells with chromosomes; with DNA an average of only 60 transformants were observed. In general, DNA transformants grown in the absence of methotrexate were unstable; whereas, under the same conditions about 20% of the transformants from the chromosome experiments were stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01542787
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation and properties of some cardopolyamides (1981)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 19 (1981), S. 2275-2285 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Several polyamides that contained cardo units were prepared. Thus 9,9-bis(4-aminophenyl)fluorene and 9,9-bis(4-aminophenyl)anthrone were condensed with 2,5-dimethyl-1,4-benzenediacetyl chloride, 2,4-dimethyl-1,4-benzendiacetyl chloride, and 2,5-dimethoxy-1,4-benzediacetyl chloride. Lowtemperature solution polycondensation in dimethyl acetamide (DMAc) was used throughout. The polymers were obtained in 80-90% yield and possessed intrinisic viscosities in the range of 0.6-1.2. The polymers were characterized by infrared (IR) spectra and elemental analysis. The solubility, crystallinity, and thermal stability of the polyamides were also determined.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1981.170190913
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  • 8
    Electronic Resource
    Electronic Resource
    Preparation and properties of polybenzimidazoles containing azo groups (1982)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 20 (1982), S. 1145-1150 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1982.170200423
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  • 9
    Electronic Resource
    Electronic Resource
    Preparation and properties of polybenzimidazoles containing cardo groups (1982)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 20 (1982), S. 3095-3105 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Several polybenzimidazoles containing cardo groups were prepared: A cardodicarboxylic acid, 9,9-bis(4-carboxyphenyl)fluorene, and two cardotetramines, 9,9-bis(3,4-diaminophenyl)fluorene and 9,9-bis(3,4-diaminophenyl)10-anthrone. The cardodicarboxylic acid was condensed with aromatic tetramines and the cardotetramines were condensed with aromatic dicarboxylic acids. Prior to polymer synthesis two model compounds, 9,9-bis[4,(2-benzimidazolyl)phenyl]fluorene and 2,2′-diphenyl-5,5′-(9,9-fluorenediyl)-bibenzimidazole were prepared and characterized by spectral methods. The polymers were obtained in 60-70% yield and showed reduced viscosity in the range of 0.7-1.1 dL/g. They were soluble in dimethyl formamide (DMF) and chlorinated solvents like tetrachlorethane. The thermal stabilities of these cardopolymers were superior to noncardopoly-benzimidazoles.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1982.170201105
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  • 10
    Electronic Resource
    Electronic Resource
    Quantitative 15N NMR spectroscopy (1980)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 14 (1980), S. 129-132 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Line intensities in 15N NMR spectra are strongly influenced by spin-lattice and spin-spin relaxation times, relaxation mechanisms and experimental conditions. Special care has to be taken in using 15N spectra for quantitative purposes. Quantitative aspects are discussed for the 15N NMR of molecules with different nitrogen functional groups and also mixtures of nitrogen-containing compounds. It is shown that, in general, quantitative data are obtainable from integration of 15N lines in proton decoupled 15N NMR spectra using NOE suppression. Addition of paramagnetic relaxation reagents (PARR) under controlled conditions is frequently needed to accomplish the experiment within reasonable time limits.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270140210
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