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  • Blackwell Publishing Ltd  (12)
  • American Institute of Physics  (10)
  • 1985-1989  (11)
  • 1980-1984  (8)
  • 1960-1964  (2)
  • 1950-1954  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Analysis of Antigens from Membrane and Soluble Fractions of Entamoeba histolytica (1986)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 33 (1986), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Axenic cultures of Entamoeba histolytica strains HK-9, HM-1, and Rahman were fractionated to provide plasma membranes, internal, vesiculated membranes, and a soluble cytosol. Each particulate fraction was solubilized and all fractions were analyzed by techniques designed to demonstrate molecular complexity and serologic reactivity. The cytosol contained more antigenic moieties than either membrane fraction; however, the antigens associated with the membranes had very high reactivity and lower nonspecific activity than the cytosol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1986.tb05619.x
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  • 2
    Electronic Resource
    Electronic Resource
    Isoenzyme Analysis of Babesia microti Infections in Humans (1986)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 33 (1986), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . We used high-resolution polyacrylamide gradient gel electrophoresis (PGGE) to separate four babesial enzymes to aid in the identification of two Babesia microti isolates established in hamsters. The isolates were compared to two different hamster passages of the “Gray” strain. All isoenzyme patterns from the two isolates and the “Gray” strain were similar except glucose phosphate isomerase (GPI) from one of the “Gray” strain passages. It showed a polymorphic GPI pattern as opposed to a monomorphic GPI pattern seen in the other “Gray” strain passage and the two isolates. The observed differences suggested (i) that some populations of B. microti are capable of having polymorphic GPI, (ii) that the “Gray” strain originally contained (and may still contain) a heterogenous population of B. microti, and (iii) that the population possessing polymorphic GPI was selected over that with monomorphic GPI. This information was obtained by a PGGE method that eliminated hemoglobin from gels and allowed, for the first time, detection of babesial leucine amino peptidase (LAP) and isocitrate dehydrogenase (IDH). In addition, this method provided molecular weight estimations on babesial GPI, LAP, IDH, and glutamate dehydrogenase (GDH), and it proved useful in the identification and characterization of the B. microti isolates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1986.tb05592.x
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  • 3
    Electronic Resource
    Electronic Resource
    High-Resolution Polyacrylamide Gradient Gel Electrophoresis (PGGE) of Isoenzymes from Five Naegleria Species (1988)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 35 (1988), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . High-resolution polyacrylamide gradient gel electrophoresis (PGGE) was used to separate isoenzymes of 12 Naegleria strains: one N. australiensis, two N. lovaniensis, one N. jadini, two N. gruberi isolated from environmental samples, and six N. fowleri strains isolated from patients with primary amoebic meningoencephalitis. Of the eight enzymes studied, seven showed zymograms with interspecific variation that identified all the species tested. Although the six N. fowleri strains were biochemically the most homogeneous, they showed intraspecific isoenzyme variation that allowed them to be grouped into four zymodemes. The PGGE technique, which separates isoenzymes by their molecular shape, is both sensitive and economical. It offers an addition or an attractive alternative to isoelectric focusing which has commonly been used to aid species identification of Naegleria by separating isoenzymes by their isoelectric point.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1988.tb04070.x
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  • 4
    Electronic Resource
    Electronic Resource
    A Fast Electrophoretic Isoenzyme Technique for the Identification of Invasive and Non-Invasive Entamoeba histolytica and “E. hitolytica-like” Organisms (1987)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 34 (1987), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cellulose acetate electrophoresis (CAE) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive Entamoeba histolytica and “E. histolytica-like” organisms. Each of these morphologically similar organisms possessed a unique CAE isoenzyme profile that can be used as an aid in their identification. The CAE technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither specialized training nor claborate equipment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1987.tb03169.x
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  • 5
    Electronic Resource
    Electronic Resource
    Limestone Influences on Physical and Chemical Features of a Mountain Stream (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 24 (1986), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Influences of limestone on physical and chemical characteristics of Abrams Creek in Cades Cove, Great Smoky Mountains National Park, were investigated from February to July 1977. Infiltration of stream water into subsurface permeable limestone conduits caused reduced and intermittent surface flow within the middle reach of the Cove. At the downstream (west) edge of the Cove, inflowing waters characteristically warmed the stream in winter and cooled it in summer and increased stream conductivity, total hardness, and pH. Management activities in the Cove did not appear to appreciably affect the chemistry of the aquatic system downstream relative to the large effects of limestone substrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1986.tb00992.x
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  • 6
    Electronic Resource
    Electronic Resource
    The Australian Register: Current Research and Future Role (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 541 (1988), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1988.tb22237.x
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  • 7
    Electronic Resource
    Electronic Resource
    Chambers and the Development of Accounting Theory: A Personal Reminiscence (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Abacus 18 (1982), S. 0 
    Details
    ISSN: 1467-6281
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1467-6281.1982.tb00034.x
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  • 8
    Electronic Resource
    Electronic Resource
    Parasitic castration of Penaeus semisulcatus as a potential source of bias in length-based stock assessments (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 19 (1988), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.1988.tb00336.x
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  • 9
    Electronic Resource
    Electronic Resource
    A METHOD TO COLLECT AND PROCESS SKIN BIOPSIES FOR CELL CULTURE FROM FREE-RANGING GRAY WHALES (ESCHRICHTIUS ROBUSTUS) (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 4 (1988), S. 0 
    Details
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: For researchers studying mysticere whales few methods for determining gender or for collecting biochemical and genetic information from unrestrained animals are available. The objective of this study was to develop a reliable method for collecting viable tissue samples for establishing continuous cell cultures from skin biopsies of free-ranging whales. A method to collect and process these samples is presented. Six of seven skin biopsies from gray whales were established in cell culture. Our results suggest that the viability of the samples is improved by (1) sterile processing in the field, (2) minimizing the time between collection and delivery to the cell culture facility, (3) reducing the concentration of antifungal agent, and (4) placing tissue explants under a coverslip. While the results reported in this paper are based on a small sample size, we believe that if the procedures are followed, they will increase the probability of successfully culturing cetacean tissue. Established cell lines can supply replenishable material from identified whales still living in the wild. These cultures can then be used for determination of sex from karyotypes, and for assessing genetic relationships of cetaceans from inherited protein, chromosomal and DNA polymorphisms. These much needed analytical tools can be used to determine familial and populational relationships, leading to a better understanding of mating systems, stock identification and effective population sizes of wild cetaceans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1748-7692.1988.tb00178.x
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  • 10
    Electronic Resource
    Electronic Resource
    The incidence of Livoneca sp. (Isopoda) on Helotes sexlineatus (Pisces) in Kuwait waters (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of applied ichthyology 3 (1987), S. 0 
    Details
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Infestations of Livoneca sp. occur on the striped therapon, Helotes sexlineatus, in Kuwait waters. Infestation rate decreases as depth increases, and some infested fish were found in all samples examined; the occurrence of infested fish could not be used as a biological tag. One year and four year old fish were uninfested. Two and three year old fish showed different infestation rates (4.1% and 0.9 % respectively). No striped therapon carried more than one parasite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0426.1987.tb00467.x
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