ISSN:
1476-5535
Keywords:
Sterilization
;
Bioreactor
;
Media
;
R0
;
F0
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Sterilization of bioreactor media, to destroy viability of the indigenous microbial population, is normally accomplished by autoclaving, or heating with pressurized steam. However, simultaneous chemical changes in media can also be expected to result from the high temperatures. A kinetic procedure involving on-line computer calculation of heat input, designated asF 0 values, was previously developed to estimate sterility achievement. A similar kinetic procedure, based on a general purpose Arrhenius ‘pseudo’ rate equation and designated asR 0 values, has now been designed to evaluate, and control the effects of temperature and heating time on chemical reactions occurring in the media. Data are presented indicating thatR 0 may be a useful parameter for reducing variability in culture metabolism and ‘scale-up’ when these variations result from different nutrient concentrations produced by non-standard heating during media sterilization in stirred bioreactors.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01574082
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