ISSN:
1612-1112
Keywords:
Ion-pair reversed-phase HPLC
;
Protoporphyrin
;
Zinc protoporphyrin
;
Red blood cells
;
Porphyria
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary An extraction and ion-pair reversed-phase HPLC procedure is described for the separation and determination of protoporphyrin and its zinc chelate from red blood cells. The recoveries were greater than 90% with coefficients of variation between 8 and 10%. A linear response curve was obtained from 800 to 4000 μg/l erythrocytes for the porphyrins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02258897
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