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  • 1990-1994  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Genealogical Identification of Hexaploid Tritordeum by Electrophoretic Separation of Endosperm Storage Proteins (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 111 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hexaploid Tritordeum (×Tritordeum Ascherson et Graebner) has been analyzed by SDS-PAGE, to test the efficiency of the method for confirming the parents of different lines, and for unequivocally discriminating between the lines. The results clearly show that endosperm storage proteins from both parents (durum wheat ——Triticum turgidum conv. durum Desf. em. M.K. —— and Hordeum chilense Roem. et Schulz.) are expressed in the endosperm of the amphiploid between them. Although the spectra of proteins from the parental species overlap on the gel, there are sufficient number of bands with distinct electrophoretic mobilities to identify unambiguously their parental origin. Furthermore, in the six lines of tritordeum analyzed, obtained from crosses between three accessions of H. chilense and four cultivars or lines of wheat, there was sufficient polymorphism amongst the parents, particularly amongst the H. chilense accessions, to enable both aims of the project to be met, that is, to confirm the genealogy of each tritordeum, and to discriminate between different lines. This study provides the basis of future investigation on the relationship between bread-making quality in tritordeum and the allelic composition of storage proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00624.x
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  • 2
    Electronic Resource
    Electronic Resource
    Screening for Greenbug Resistance in Hordeum chilense Roem et Schult. (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 112 (1994), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Schizaphis graminum (Rondani) is a serious pest of cultivated wheat (Triticum aestivum L.) and resistance is only available in other related species such as Hordeum chilense. Amphiploids between H. chilense and Triticum spp. have been obtained, and addition lines of H. chilense in wheat have been developed.Thirty-five accessions of H. chilense were screened to identify greenbug antixenosis, antibiosis and tolerance. Antixenosis was determined in a conventional host free choice test; antibiosis was measured by aphid life cycle and fecundity rate, and tolerance was tested in a conventional infestation test of 4 weeks. Two commercial barley cultivars were used as susceptible and resistant controls.Eight H. chilense accessions showed higher degrees of antixenosis than the resistant check, 19 were similar and the rest were lower. All accessions were more resistant than the susceptible check. Measured by aphid life cycle, 22 H. chilense accessions showed higher antibiosis than the resistant cv., and all exhibited a higher antibiotic effect on fecundity rates than the control. A similar degree of tolerance to that of the resistant control was observed in six accessions, the remaining entries ranged between the controls. The presence of one mechanism did not exclude the existence of other mechanisms in the same entry and therefore, independence of the different mechanisms is proposed.Most of the accessions showed higher variability than both controls for the three mechanisms, and it appears to be genetic variability within entries for the three mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1994.tb00663.x
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