ISSN:
1573-5044
Keywords:
dark
;
in vitro storage
;
light
;
stability
;
temperature
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In vitro cultures of Nephrolepis exaltata and Cordyline fruticosa were stored at 5°, 9° or 13°C, at a low irradiance (3–5 μmol m−2 s−1) or in darkness. Prior to storage the cultures were subjected to 18°, 21°, 24° or 27°C and 15, 30 or 45 μmol m−2 s−1 in a factorial combination. The optimal storage conditions for Nephrolepis were 9°C in complete darkness. These cultures were still transferable to a peat/perlite mixture at the end of the experimental period of 36 months. The optimal storage conditions for Cordyline were 13°C and a low light level (±3–5 μmol m-2 s-1). When the pre-storage conditions were normal growth room conditions (24°C and 30 μmol m-2 s-1), in vitro cultures could be stored for 18 months. With the most favourable pre-storage treatment (18°C and 15 μmol m-2 s-1) some cultures still had green shoots after 36 months of storage, but did not survive transfer to peat/perlite. Pre-conditioning before storage was most favourable for Nephrolepis, and not that important, but still favourable, for Cordyline. There was an interaction between pre-storage temperature and pre-storage irradiance. For both species a high irradiance level was less favourable than a low irradiance level when combined with high growth room temperatures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00055513
Permalink
