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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 48 (1993), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Seven cultivars of white clover (Trifolium repens L.) (Kent, S184, Huia, Menna, Donna, Alice and Nesta) and a commercial mixture, ‘Ensign’, were strip-seeded into an upland perennial rye-grass (Lolium perenne L.) sward in late June 1986. Swards were first grazed by sheep, either on 5/6 August (early) or on 19/20 August (late) and then every 14–21 days (frequently) or 28–42 days (infrequently) during 1986, followed by a common grazing regime in 1987. During April to mid-June 1988 the swards received either a moderate amount of nitrogen or none and were cut frequently or once only in mid-June. Growth of individual seedlings was assessed before and after grazing during 1986 and stolon accumulation and distribution and sward colonization were assessed during 1987 and 1988.All cultivars emerged rapidly and satisfactorily and there were no consistent significant differences in the overall dry matter accumulation per seedling during establishment. During the first autumn the proportion of the aboveground biomass removed during grazing was smallest in Kent (c. 20%) and largest in Nesta (c. 40%). Kent and S184 produced most leaves and stolons and the greatest length of stolons per seedling and per individual stolon, and Nesta and Alice the fewest leaves and stolons and shortest stolons. Seedlings grazed early had heavier and longer stolons than those grazed late; those grazed frequently had more leaves, stolons and growing points than those grazed infrequently, especially following early grazing.During 1987 Kent and S184 had consistently the largest number of stolon growing points, and weight and length of stolons per unit area; these two cultivars and Nesta also colonized the sward more rapidly than the other cultivars. All cultivars contributed substantially and similarly to herbage production in late September. There were no residual effects of the 1986 treatments after the summer of 1987.During 1988 additions of nitrogen fertilizer at 100 kg N ha-1 or allowing the herbage to remain undefoliated between mid-April and mid-June both independently halved the number of stolon growing points per unit area; together they reduced it by 80%. Nitrogen also, on average, halved stolon weights but less so in Nesta, Alice and Huia and more so in all other cultivars. Infrequent defoliation greatly decreased stolon weights in Kent and S184 but had no significant effects on the other cultivars. Sward colonization was almost complete by June and entirely so by October for all cultivars in all treatments.Implications of the results for the after-management of strip-seeded white clover are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Grass and forage science 45 (1990), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Small plots of a Festuca-Agrostis upland sward on a peaty gley podsol were strip-seeded during late June 1986 with white clover cvs Aberystwyth S184 or Menna at 4 kg ha−1 and defoliated early (20 August) or late (3 September) and then frequently or infrequently (every 2 weeks or 4 weeks) until the end of September. All plots were defoliated in early November, at 3-weekly intervals during the growing season in 1987 and then grazed rotationally during 1988.Satisfactory seedling establishment, representing 46% emergence, was achieved 5 weeks after sowing. The differential defoliation regimes had no persistent significant effects on clover development. S184 soon produced more leaves per seedling than Menna and a smaller proportion of its leaf number and weight were removed at each defoliation. Following large losses of leaves over the 1986–87 winter, SI84 had significantly more leaves per stolon than Menna; subsequently it also colonized the sward at a quicker rate. During 1987 amounts of herbage harvested (6.1 t ha−1) were similar with the two clover cultivars, with S184 contributing 47% and Menna 44% of this respectively. SI84 made a larger contribution to yield during May and June but Menna was more productive during September and October. During 1988 clover populations were maintained with rotational grazing without additional fertilizer inputs.The results show that, despite initial soil and climatic contraints, both small and medium-leaved clovers can be strip-seeded into upland swards with large subsequent benefits to yield and herbage quality. However, they also indicate the need for further experiments to determine the influence of sward morphology and defoliation regime on stolon branching rates and accumulation of growing points which, in turn, govern sward colonization.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An analytical method has been developed and validated for the quantitative determination of the N-methyl-D-aspartate (NMDA) antagonist cis-4-phosphonomethyl-2-piperidine carboxylic acid (CGS 19755) in human plasma. It is a member of a new class of compounds with the potential to be neuroprotective and attenuate neuronal damage resulting from brain trauma caused by stroke and head trauma. The method is based on gas chromatography/mass spectrometry and uses stable-isotope labeled CGS 19755 as the internal standard. Samples (1 ml) were first acidified (pH 2), then extracted using a solid-phase aminopropyl ion exchange column. The drug was eluted with NH4OH and evaporated until dry. Extracts were derivatized with a mixture of pentafluoropropionic anhydride and pentafluoropropanol, and analyzed by gas chromatography/mass spectrometry. Separation was accomplished on a DB-225 capillary column (15 m × 0.32 mm) with a 0.25 μ film thickness. Mass spectrometry was carried out under negative ion ammonia chemical ionization conditions with selected ion monitoring at m/z 760 and 764 for derivatized CGS 19755 and the internal standard, respectively. Specificity was shown by the lack of interfering peaks at the retention time of CGS 19755 and internal standard. Recovery and reproducibility assessments show good accuracy, precision and linearity over the validated concentration range of 2-5000 ng ml-1.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 1991-08-01
    Description: SUMMARYSpring barley and spring oats were strip-seeded into crops of the white clover cultivar Alice at Hereford in 1987 and cultivar S184 at Aberystwyth in 1988. Drilling was done with or without a band-spray of glyphosate, a moderate or low (1988 only) dose of paraquat or into plots where the clover had been killed by herbicide 2 months previously.In the first experiment, initial cereal emergence was sparse; growth was suppressed in the unchecked (unsprayed) clover base but was vigorous in the clover-free plots; the cereals also became dominant in the swards sprayed with herbicide. Whole-crop yields in mid-August were 13, 3–4 and 8–10 t DM/ha in the plots in which clover was killed, unchecked or checked with herbicide. Clover contributed 4–12% of the harvested herbage in the latter treatment. This treatment also yielded c. 70 % as much N, carbohydrate and fibre as that without clover. Grain yields exceeded 7 t/ha without clover but were only 0·3 t/ha for barley and 1·1 t/ha for oats with unchecked clover; in the checked clover plots, barley yielded 60% and oats 78% as much as on the clover-free plots. Four and 5 weeks after whole-crop harvest, residual clover growth was 27 and 39% of that on unchecked plots for oats and barley, respectively.In the second experiment, the cereals emerged thickly but were later dominated by the clover, and an equitable balance was achieved only with the larger dose of paraquat. However, the oat cultivar Emrys was suppressed less than the tall barley cultivar Dandy; the short barley cultivar Digger was the most suppressed. Mean whole-crop yields were 11 t/ha in the treatment without clover, about half this in the unchecked bases and c. 9 t/ha with the larger dose of paraquat. Differences in chemical composition reflected much larger clover contents in 1988 than in 1987. Yields of N and water-soluble carbohydrate were at least as large or larger with moderate paraquat than for the clover-free plots. Grain yields ranged from 5·6 to 6·9 t/ha for the three cereal cultivars without clover but were negligible to very small in the unchecked and band-sprayed treatments, and were 3·4 and 2·0 t/ha for oats and barley, respectively, with the larger dose of paraquat. Residual stolon weights, 70–80 days after whole-crop harvest, greatly exceeded initial values in all treatments. They were smallest following the larger dose of paraquat, and larger in Digger than in Dandy, which in turn was larger than in Emrys.It is concluded that the concept of growing cereals in a clover base shows potential as a low input–moderate output system of cereal production. However, further longer term work is needed on regulation of the cereal–clover balance, on the release and uptake of N and the environmental effects of the technique.
    Print ISSN: 0021-8596
    Electronic ISSN: 1469-5146
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 5
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