ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The gene encoding the archaeal 7S RNA in the hyperthermophile Methanothermus fervidus is linked to a tRNASer and rRNA operon in the arrangement 5′-7S RNA-14nt-tRNASer-196nt-16S rRNA and the promoter directing transcription of this 7S RNA gene has now been identified. Initiation of transcription of the 7S RNA gene has been shown to occur both in vivo in M. fervidus and in vitro, using a Methanococcus thermolithotrophicus derived cell-free transcription system, at the first G residue within the initiator sequence ATGG, located 6 bp upstream of the 5′ end of the 7S RNA coding region. Cotranscription of the 7S RNA and tRNASer has been demonstrated in vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1992.tb05496.x
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