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  • 1
    Electronic Resource
    Electronic Resource
    Low rf-loss, bondable ohmic contacts on Tl2Ba2CaCu2O8−δ for microwave devices (1993)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 73 (1993), S. 3092-3095 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We present two processes for the fabrication of bondable, low-loss ohmic contacts to high-temperature superconductor thin-film devices. Contact resistances at both high and low frequencies are comparable—between 5.0×10−7 and 2.0×10−5 Ω cm2 at 77 K. We demonstrate that losses at low frequency correlate with losses up to 10 GHz. Destructive bond pull strengths for 25-μm-diam, thermosonically attached gold wire average between 5.0 and 8.0 g force, exceeding the industry standard of 3.0 g force. Preliminary results also show that contact resistance as low as 1.7×10−8 Ω cm2 are attainable with annealing. These processes satisfy a pressing need for fabrication of reliable, low-loss ohmic contacts required for the development and production of high-performance passive microwave devices based on high-Tc superconducting thin-film materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.352994
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  • 2
    Electronic Resource
    Electronic Resource
    Proper and Improper Folding of Proteins in the Cellular Environment (1991)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 45 (1991), S. 607-635 
    Details
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.mi.45.100191.003135
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of receptor agonists on polyamine concentrations and spermidine/spermine N1-acetyltransferase activity in rat parotid gland (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 881-884 
    Details
    ISSN: 1420-9071
    Keywords: Epidermal growth factor ; forskolin ; isoprenaline ; pilocarpine ; polyamines ; spermidine/spermine N1-acetyltransferase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The polyamine putrescine might be formed via a degradation (catalyzed by spermidine/spermine N1-acetyltransferase, SSAT) of the higher polyamines spermidine and spermine to putrescine. The involvement of different intracellular signal pathways in the regulation of putrescine formation was studied in explants and in cultured cells of rat parotid glands by using receptor agonists that activate separate second messenger systems, and measuring their effects on the concentrations of putrescine, spermidine and spermine and on the SSAT activity. The β-adrenoceptor agonist isoprenaline, which is an activator of cAMP formation, increased the putrescine concentration and stimulated the SSAT activity. Pilocarpine, a drug that activates the muscarinic receptors and thereby enhances the phosphoinositide turnover, had no effect on either the polyamine concentrations or on the SSAT activity. Epidermal growth factor (EGF), which induces activation of a protein tyrosine kinase, had no effect on the polyamine concentrations or on the SSAT activity. The adenylate cyclase activator forskolin increased the glandular levels of putrescine. Taken together, these findings suggest that increases in putrescine concentration in cultured rat parotid gland cells are accompanied by accumulation of cAMP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952602
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  • 4
    Electronic Resource
    Electronic Resource
    String vertices, overlap equations, τ functions and the Hirota equation (1992)
    Springer
    Communications in mathematical physics 145 (1992), S. 329-344 
    Details
    ISSN: 1432-0916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Abstract String vertices,V, are shown to satisfy a new type of overlap equation of the form $$V \exp \{ ip \cdot Q^i (\xi ^i )\} = V \exp \{ ip \cdot Q^i (\xi ^i )\} \left( {\frac{{d\xi ^j }}{{d\xi ^i }}} \right)^{p^2 /2} $$ as well as corresponding equations forA n andB n cycles. A special case of such an equation, when integrated, is shown to be the Hirota equation for the K−P hierarchy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02099140
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  • 5
    Electronic Resource
    Electronic Resource
    Relative absorption of the two enantiomers of terbutaline after duodenal administration (1990)
    Springer
    European journal of clinical pharmacology 38 (1990), S. 621-623 
    Details
    ISSN: 1432-1041
    Keywords: terbutaline ; absorption ; enantiomers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The absorption of the two enantiomers of terbutaline, (+)-terbutaline and (-)-terbutaline, and of the racemate, (±)-terbutaline, has been studied in six healthy volunteers using a newly developed intestinal perfusion technique. The area under the plasma concentration curve for the (-)-enantiomer was more than twice that for the (+)-enantiomer. The data demonstrate a clear difference in absorption efficiency between (-)- and (+)-terbutaline, but further studies are required to establish whether (-)-terbutaline influences the absorption of (+)-terbutaline.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278593
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  • 6
    Electronic Resource
    Electronic Resource
    S19.4 Mass spectrometry of glycoconjugates after specific chemical modifications (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 335-335 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01210158
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  • 7
    Electronic Resource
    Electronic Resource
    S9.31 Minor carbohydrate structures responsible for binding of some lectins by glycophorin A (1993)
    Springer
    Glycoconjugate journal 10 (1993), S. 285-285 
    Details
    ISSN: 1573-4986
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01210003
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  • 8
    Electronic Resource
    Electronic Resource
    Carbohydrate antigen expression in murine embryonic stem cells and embryos. II. Sialylated antigens and glycolipid analysis (1993)
    Springer
    Journal of molecular histology 25 (1993), S. 628-641 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A mouse embryonic stem (ES) cell line E14 and early mouse embryos were stained with a panel of 15 monoclonal antibodies recognizing sialylated or potentially sialylated carbohydrate determinants, Sialyl Le-x and sialyl Le-a were detected on the pre-implantation embryo from the 8-cell stage, and sialyl Le-a weakly on undifferentiated ES cells. Changes in cell surface carbohydrates occurred after induction of ES cell differentiation with retinoic acid (RA) and dibutyryl cAMP. Qualitative analysis of the neutral glycolipids of untreated and RA-treated ES cells using high-performance thin-layer chromatography (HPTLC) revealed few differences between the two types of culture. The major gangliosides in both cultures were indicative of an active ‘a’ ganglioside synthesis pathway. GD3, a precursor of the ‘b’ synthesis pathway, previously reported to be characteristic of embryonal carcinoma (EC) cells, was absent. RA-induced differentiation caused a shift in the spectrum to more complex gangliosides. Application of fast atom bombardment mass spectrometry (FAB-MS) to permethylated derivatives of individual bands permitted partial characterization of an unusual sialylated glycolipid and a rare ganglioside with the suggested structure of GalNAc-GD1a.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00157877
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of a novel lectin inStreptococcus pyogenes and its possible role in bacterial adherence to pharyngeal cells (1994)
    Springer
    Current microbiology 28 (1994), S. 331-338 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract During investigation of the interaction of human lactoferrin (HLf) with variou bacteria, it was found that inStreptococcus pyogenes, HLf binding occurred to agar-rather than broth-grown cells irrespective of the nutrients used. Furthermore, binding of HLf to broth-grown, heat-killed bacteria was induced by overnight incubation on agar media or short-time exposure of the cells to water-soluble agar extract. The binding pattern was revealed in most of 92S. pyogenes strains representing various M-or T-types with no apparent type variation. The component thus bridging the attachment of HLf to the streptococcal cell surface was recovered in extracts of agar-grown cells and isolated by affinity chromatography on HLf-sepharose. By gel filtration in the presence of radiolabeled HLf, this component exhibited similar elution position as crude water-soluble agar extract. Chemical analysis identified the active HLf-binding agar component to be a galactose-rich polysaccharide (GRP). Further binding tests showed that the interaction between streptococci and GRP was stable in the presence of high molar NaCl, KSCN, or urea and was unaffected by various serum or matrix proteins or by streptococcal lipoteichoic acid; however, a moderate inhibition by heparin or bovine mucin was observed. Studies on isogenic mutants ofS. pyogenes did not support the involvement of M-protein or the hyaluronate capsule in the binding of GRP. SDS-PAGE and Western blot analyses revealed a GRP-binding protein of approximately 70 kDa in the cell-wall extracts of two strains ofS. pyogenes, types M19 and M55. Finally, the adherence of (broth-grown)3H-thymidine-labeledS. pyogenes, type M19, to the pharyngeal epithelial cell line DT-562 or to normal tonsillar epithelial cells was inhibited by GRP in a dose-related manner. We thus propose that the streptococcal GRP-binding component may represent a novel surface lectin acting as a mucosal adhesin forS. pyogenes, in accordance with previous data indicating that galactosecontaining sugar moieties may serve as ligands for the adherence of streptococci to pharyngeal cells. Our results also indicate that GRP-like components such as mucin or heparin might act to block epithelial adherence ofS. pyogenes at the mucosal level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570197
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of mouse-hamster-human cross-reacting antioocyte monoclonal antibodies produced by intrasplenic immunization of mice with 12 zona-free mouse oocytes (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 37 (1994), S. 446-451 
    Details
    ISSN: 1040-452X
    Keywords: In vitro fertilization ; Fertilization-specific antibody ; Immunohistochemistry ; Mouse-hamster-human cross-reacting antibodies ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Several intrasplenic immunizations with batches of ∼15 or ∼30 zona-free, unfertilized mouse oocytes resulted in 200-300 hybrids, respectively, among which about 20 positive clones were selected from each fusion between splenic plasma cells and SP2/0 myeloma cells. When nonimmunized splenic plasma cells were used, only one antibody, showing weak immunoreaction, was obtained from ∼370 hybrids collected from 2 fusions. From one immunization with a total of 12 zona-free, unfertilized mouse oocytes, 15 positive clones were selected for further study. Eleven of these 15 antibodies reacted with antigens only in unfertilized oocytes but not in fertilized, pronuclear stage oocytes. Three antibodies, which recognized antigens in paraffin-embedded oocyte sections, did not label growing ovarian oocytes, indicating that the antibodies were specific to ovulated, unfertilized oocytes. These antibodies did not detect any antigen epitopes in the panel of tissues examined. The molecular weight of one antigen, corresponding to a IgM antibody that is present both in ooplasma and zona pellucida, was ∼116 kDa. Cross-reactivity to blots of unfertilized zona-free hamster oocytes was demonstrated by 6 antibodies and to unfertilized human oocytes by 7 antibodies. Three antibodies cross-reacted with both hamster and human oocytes. The study indicates that the intrasplenic immunization is an appropriate means of raising antibodies against unfertilized, zona-free mouse oocytes and that the method applied offers an easy way to select antibodies against human oocytes for functional studies. © 1994 Wiley-Liss, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080370411
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