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Human AT1 receptor is a single copy gene: Characterization in a stable cell line (1994)

Aiyar, Nambi ; Baker, Elayne ; Wu, Hsiao-Ling ; [et al.]

Springer

Molecular and cellular biochemistry 131 (1994), S. 75-86

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ISSN: 1573-4919

Keywords: angiotensin II receptor ; gene ; stable cell line ; human

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Abstract To address conflicting reports concerning the number of angiotensin II (AII) receptor type 1 (AT1) coding loci in vertebrates, Southern blot analysis was used to determine the genomic representation of AT1 receptor genes in animals comprising a divergent evolutionary spectrum. The data demonstrate that the AT1 receptor gene is present as a single genomic copy in a broad spectrum of animals including human, monkey, dog, cow, rabbit, and chicken. In contrast, members of the rodent taxonomic order contain two genes in their genomes. These two genes may have arisen in rodents as a consequence of a gene duplication event that occurred during evolution following the branching of rodents from the mammalian phylogenetic tree. In order to investigate the properties of the human AT1 receptor in a pure cell system, the recombinant human AT1 receptor was stably expressed in mouse L cells. An isolated cell line, designated LhAT1-D6, was found to express abundant levels of recombinant receptor [430±15 fmol/mg] exhibiting high affinity [KD=0.15±0.02 nM] for [125I][SAR1, IIe8] angiotensin II (SIA). The pharmacological profile of ligands competing for [125I] SIA binding to the expressed recèptor was in accordance with that of the natural receptor. Radioligand binding of the expressed receptor was decreased in the presence of the non-hydrolyzable analog of GTP, guanosine 5′-(γ-thio) triphosphate [GTPγS]. Angiotensin II evoked a rapid efflux of45Ca2+ from LhAT1-D6 cells that was blocked by AT1 receptor specific antagonists. In addition, AII inhibited forskolin-stimulated cAMP accumulation in these cells which was blocked by the AT-1 antagonist. Thus, the LhAT1-D6 cell line provides a powerful tool to explore the human AT1 receptor regulation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01075727

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Foot currents and ankle SARs induced by dielectric heaters (1992)

Conover, David L. ; Moss, C. Eugene ; Murray, William E. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Bioelectromagnetics 13 (1992), S. 103-110

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ISSN: 0197-8462

Keywords: dielectric heaters ; body currents ; SARs ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Physics

Notes: Data are presented on ankle-specific SARs and foot currents as a function of strengths of radio-frequency electromagnetic fields encountered by operators of dielectric heaters. The determination of foot currents was based on near-field exposures in which reactive coupling dominates, and which can result in substantial SARs in exposed workers. The operators were located less than one wavelength from - usually within one meter of - the dielectric heaters, which generated fields at frequencies from 6.5 to 65 MHz. At distances normally assumed by workers, maximal strengths of electric fields ranged from 104 to 2.4 × 106 V2/m2; maximal strengths of magnetic fields ranged from 5.0 × 10-3 to 33.3 A2/m2. Currents through both feet to ground were measured while operators stood where they normally worked. Maximal currents ranged from 3 to 617 mA, rms. Nearly 27 percent of the dielectric heaters induced foot currents that exceeded the 200-mA limit that has been proposed for a new ANSI C95.1 standard. Twenty percent of the heaters induced foot currents that exceeded 350 mA. SARs in ankles were calculated from foot currents, and they approximated 5 W/kg at 100 mA, 29 W/kg at 250 mA, and 57 W/kg at 350 mA. The maximal SAR in the ankle was ∼ 176 W/kg at 617 mA. © 1992 Wiley-Liss, Inc.

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