ISSN:
1432-072X
Keywords:
Trichoderma reesei
;
Xylanase
;
Ultrastructural localization
;
Immunogold labelling
;
Electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The intracellular location of the “low-molecular weight, alkaline” xylanase (XYN II) of Trichoderma reesei RUT C-30 was investigated during growth on xylan, using immunoelectron microscopy. A monoclonal antibody, produced against XYN II, was used for this purpose. The enzyme was found at the endoplasmic reticulum and in electron dense 0.2 to 0.8 μm vesicles, as well as in the vacuole, at the plasma membrane and in the fungal cell-wall. No staining occured in the cytoplasm, the mitochondria and the nucleus. No Golgi-like structures could be seen. Addition of the carboxylic ionophore monensin blocked xylanase as well as total protein secretion. The results are discussed with respect to XYN II being secreted by T. reesei via a pathway involving the endoplasmic reticulum and secretory vesicles and/or the vacuole.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00288587
Permalink