ISSN:
1432-2048
Keywords:
cDNA clone (sucrose synthase)
;
Gene expression (sucrose synthase, seed development)
;
Nucleotide sequence (sucrose synthase)
;
Seed development
;
Sucrose synthase (cloning, expression, regulation)
;
Vicia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Copy-DNA clones encoding a single class of sucrose-synthase (SUCS; EC 2.4.1.13) subunit have been isolated and sequenced from a Vicia faba L. seed cotyledonary library. Southern analyses indicated the existence of only one gene. Transcript levels determined by Northern blot hybridisation steadily increased until the middle of development [25–35 days after flowering (DAF)] and declined thereafter. Sucrose levels approximately paralleled levels of SUCS mRNA. The activity of SUCS increased with decreasing fructose and glucose concentrations and peaked about 10 d later than mRNA levels. In-vitro culture experiments demonstrated that increasing the sucrose concentration leads to increased levels of SUCS mRNA. The SUCS mRNA was also synthesised in seed-coat tissue, but in lower amounts than in cotyledons and with a different developmental profile. The early peak level of SUCS mRNA (20 DAF) in seed coats coincided with the peak in the amount of sucrose and with a peak of transiently synthesised starch.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00195698
Permalink
